Cysteine proteases have a common catalytic mechanism that involves a nucleophilic cysteine thiol in a catalytic dyad. The first step is deprotonation of a thiol in the enzyme's active site by an adjacent amino acid with a basic side chain, usually a histidine residue. The next step is ...
Cysteine Protease In subject area: Neuroscience Cysteine proteases are a type of proteases that use a covalent mechanism involving the formation of a thioester bond between the active site cysteine and the carboxyl group of the cleaved peptide bond. ...
EcpA is an extracellular cysteine protease protein involved in biofilm formation on medical devices. Thus, blocking this mechanism may be viable for developing a drug against S. epidermidis. The current research aimed to find new, potent inhibitors that could stop the S. epidermidis EcpA protein ...
Protease-Activated Receptors Proteases Proteasome Protein Kinase A Protein Kinase B Protein Kinase C Protein Kinase D Protein Kinase G Protein Methyltransferases Protein Prenyltransferases Protein Ser/Thr Phosphatases Protein Synthesis Protein Tyrosine Phosphatases ...
In the case of the cysteine proteases, the catalytic mechanism has been most extensively investigated for the clan CA enzymes. Despite their distinct evolutionary origins, it appears that the mechanism of other cysteine proteases is quite similar (an example of convergent evolution). Cleavage of the...
Mass spectrometry (MS)-based chemoproteomics methods are emerging as powerful approaches to expand the ligandable proteome, enabling the identification of small molecule-protein interactions in a cellular context. Small molecule ligands that act via an irreversible covalent mechanism are particularly suited...
Figure 9.10.Cysteine protease (Wikipedia). Cysteine proteasesare commonly found in fruits, such aspapaya, pineapple, fig, andkiwifruit. A good example of cysteine protease ispapain,which is found in the papaya fruit, The catalytic mechanism used by thisenzymefor thehydrolysisof the peptide bond in...
General SolidPhase Method for the Preparation of Mechanism-Based Cysteine Protease Inhibitors The first general method has been developed for the expedient solid-phase synthesis of ketone-based cysteine protease inhibitors. The synthesis approach wa... A Lee,L Huang,JA Ellman - 《Journal of the Ame...
The present invention relates to a novel polypeptide which displays IgG cysteine protease activity, and in vivo and ex vivo uses thereof. Uses of the polypeptide include methods for
Although it had been speculated previously that the proteolytic activity of Der p I might be related to its allergenicity, no explanation had been offered for the mechanism of the putative proteolytic event, nor has any candidate previously been proposed as a substrate of this proteolytic activity....