likecysteine proteasefamily (MEROPS family C1) belongs to the CA clan. These proteins are about 30kDa in size and fold into two domains that form a cleft containing the active site (Fig. 1K). Members of this protein family are found in viruses, bacteria, and eukaryotes. In plants, these...
pathological processes, from thebulkdigestion of protein, on the one hand, to highly regulated rate-determining and specific peptide-bond cleavages at the opposite extreme. They are implicated in a number of human pathologies, and the development of cysteineprotease inhibitorsas drugs is a very ...
Protease-Activated Receptors Proteases Proteasome Protein Kinase A Protein Kinase B Protein Kinase C Protein Kinase D Protein Kinase G Protein Methyltransferases Protein Prenyltransferases Protein Ser/Thr Phosphatases Protein Synthesis Protein Tyrosine Phosphatases ...
which belong to the γ-glutamylcyclotransferase family.97,98These enzymes catalyse the degradation of glutathione and the release of cysteinylglycine in the cytosol, rather than outside the cell as for GGT. Although not much is known about these enzymes in cancer, the CHAC2 isoform has already ...
Cysteine peptidases of clan CA, family C1 account for a major part of proteolytic activity in the haematophagous monogenean Eudiplozoon nipponicum. The full spectrum of cysteine cathepsins is, however, unknown and their particular biochemical properties, tissue localisation, and involvement in parasit...
After the last wash, 6,000 worms were sonicated in 100 μl of lysis buffer (50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1% (v/v) NP-40, 2 mM EDTA) containing protease inhibitor and phosphatase inhibitor cocktails (Roche), and insoluble material was removed by centrifugation for ...
Putative Cysteine Protease 339 C4MA38 Papain family CP domain containing protein 571 C4M0G5 Papain family CP domain containing protein 572 C4M3I8 Uncharacterized Protein 502 C4LSR0 Uncharacterized Protein 554 C4LYT1 Peptidase C1 domain containing protein 503 C4LWX8 Uncharacterized Protein 627 C4LV58 ...
Cathepsin S (CTSS), a lysosomal cysteine protease, has been reported to be associated with extracellular matrix (ECM) degradation, thus promoting cell migration and invasion, but whether CTSS regulates other intracellular mechanisms during metastasis remains unknown. The expression of CTSS was knocked ...
HepG2 cells were treated with CL1 at the indicated concentration for 24 h and then harvested by lysis buffer (10 mM β-glycerol phosphate, 10 mM Na4P2O7, 40 mM HEPES, 4 mM EDTA, supplemented with Protease and Phosphatase inhibitor (ThermoFisher Scientific #A32961)). The ...
Clan/Cysteineprotease/Evolution/Secondarystructure.IntroductionTheenzymesweknowascysteineproteases,orcysteinepeptidasesasweshallrefertothemhere,areoftenthoughtofasallbeingrelated,andsometimesareevenreferredtoasthe'cysteineproteasefamily',butthereal-ity is thattheyhavecomefroma numberofquitesepa-rateevolutionaryorigins....