A developmentally regulated cysteine protease gene family in Haemonchus contortus The nucleotide sequence of a gene encoding a 35-kDa thiol protease of the parasitic nematode Haemonchus contortus has been determined. The gene, designated... D Pratt,GN Cox,MJ Milhausen,... - 《Molecular & ...
Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4,150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na3VO4, 25 mM β-glycerol-phosphate, 0.1 mM PMSF, with a complete Roche protease inhibitor set and a Sigma phosphatase inhibitor set). ...
Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4,150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na3VO4, 25 mM β-glycerol-phosphate, 0.1 mM PMSF, with a complete Roche protease inhibitor set and a Sigma phosphatase inhibitor set). ...