Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4,150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na3VO4, 25 mM b-glycerol-phosphate, 0.1 mM PMSF, with a complete Roche protease inhibitor set and a Sigma phosphatase inhibitor set). The resuspended...
Cell pellets were collected and resuspended in lysis buffer (20 mM Tris-HCl, pH 7.4,150 mM NaCl, 10% glycerol, 1% Triton X-100, 1 mM Na3VO4, 25 mM β-glycerol-phosphate, 0.1 mM PMSF, with a complete Roche protease inhibitor set and a Sigma phosphatase inhibitor set). ...