In a two-hybrid screen using a bait protein to mimic phosphorylated cyclin B1, we identified a novel interaction between cyclin B1 and patched1 (ptc1), a tumor suppressor associated with basal cell carcinoma (BCC). Ptc1 interacted specifically with constitutively phosphorylated cyclin B1 derivatives...
Cited in 75 publications. Cyclin B1 antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA and reacts with human, rat.
Cyclin B1 protein was also degraded at a comparable rate. CDT1 activity is tightly controlled by proteasome6. Treatment with proteasome inhibitor increased the level of free CDT1 to a similar degree in both control and knockdown cells, but did not restore chromatin-bound CDT1 levels induced by...
Interaction between cyclin D1 and steroid receptor coactivators and users thereof in assays 来自 百度文库 喜欢 0 阅读量: 21 申请(专利)号: US19990302305 申请日期: 1999-04-30 公开/公告号: US6350572B1 公开/公告日期: 2002-02-26 申请(专利权)人: PROLIFIX LIMITED 发明人:...
Northern-blot analysis indicated that the radiation-induced decrease in cyclin B1 protein expression was accompanied by a reduced cyclin B mRNA level. On the whole, our results indicate a direct inhibitory effect of 10 Gy irradiation on cyclin B1 expression as a possible cause for the persiste...
We proposed that the investigated F-box protein, as a part of the SCF complex, probably not directly but through other target, contributes to the degradation of CYCB1;1. Additional analyses of protein levels are needed to identify the main ubiquitination target/s of the cyc...
Recently, a new class of E3 ligases referred to as SCF complexes has been identified that consists of four subunits:SKP1, Cul1, Roc1 and an F-box protein, the latter of which determines substrate specifity. We previously reported the cloning of NIPA (nuclear interaction partner of ALK) in ...
a In vitro kinase assay using active recombinant Cyclin B1/CDK1, Cyclin A2/CDK2, Cyclin E1/CDK2, Cyclin D1/CDK4 or Cyclin D1/CDK6 (as indicated) on purified DENR·MCTS1 protein complex and analyzed by Western Blot shows phosphorylation of DENR Ser73 by Cyclin B1/CDK1 and Cyclin A2/...
We report that CDK1-cyclin B1 phosphorylates the RNMT regulatory domain on T77 during G2/M phase of the cell cycle. RNMT T77 phosphorylation activates the enzyme both directly and indirectly by inhibiting interaction with KPNA2, an RNMT inhibitor. RNMT T77 phosphorylation results in elevated ...
Phosphorylation of serine residues in the NES (by kinases including CDK1 and PLK1) is important for its nuclear translocation, possibly through the disruption of CRM1–cyclin B1 interaction. This mechanism allows the localization of cyclin B1–CDK1 to the nucleus when the complexes are active. ...