The atomic coordinates and structure factors were deposited to the PDB (www.pdb.org) under accession codes 8VQ3 (I-198–CCNE1:CDK2 structure) and 8VQ4 (I-125A–CCNE1:CKD2 structure)). Coding sequences of the DNA constructs used in this study are provided in Supplementary Table 1. ...
The cell cycle is driven by the action of cyclin-dependent kinases (CDKs) (Hartwell and Kastan, 1994; Malumbres and Barbacid, 2001; Nurse, 2012; Sherr, 1996). Conventionally, D-type cyclins (cyclins D1, D2, or D3) accumulate in response to physiological mitogenic signals or oncogenic ...
Cyclin-CDK complexes are in turn regulated by the cyclin-dependent kinase inhibitors (CDKIs), which generally inhibit cell cycle progression (Table 1). These proteins fall into two families based on their structural and functional properties. The INK4 group includes p16/INK4A (p16), p15/INK4B...
Cell cycle transitions result from global changes in protein phosphorylation states triggered by cyclin-dependent kinases (CDKs). To understand how this complexity produces an ordered and rapid cellular reorganisation, we generated a high-resolution map of changing phosphosites throughout unperturbed early...
The relationship between the topochemical indices and cyclin-dependent kinase 2 (CDK2) inhibitory activity of indole-2-ones has been investigated. The relationship of topochemical versions of well known topological indices of Wiener's index--a distance-based topological descriptor, molecular connectivity...
Cyclin-dependent kinase 11 (CDK11), which plays a pivotal role in cell cycle regulation and transcription, is overexpressed in various cancers and is linked to poor prognosis. This study focused on identifying potential inhibitors of CDK11 using computational drug discovery methods. Techniques such ...
The cells infected with the e-f-r- virus, lacking both Vif and Vpr, exhibited similar nuclear translocation of Cdk1 as the mock-infected cells, but with delayed kinetics (Figure 2Aa-c and 2B). Thus, our data suggest that Vif inhibits Cdk1 nuclear translocation whether or not Vpr is ...
CCND1 mRNA expression and IHC staining in NPC patients and PDX tumors. (a) The expression fold change of candidate genes (CCND1, CDKN2A and CDKN2B) are indicated based on the cDNA microarray data of five PDX tissues, and C666–1 (EBV-positive NPC cells) and NP69 (immortalized normal ...
Together with its binding partners cyclin dependent kinase 4 and 6 (CDK4 and CDK6), cyclin D1 forms active complexes that promote cell cycle progression [12]. Cyclin D1 is important for the development and progression of several cancer types, including that of oral epithelial cancer that ...
Cyclin/cyclin-dependent kinase (CDK) complexes are critical regulators of cellular proliferation.A complex network of regulatory mechanisms has evolved to control their activity, including activating and inactivating phosphorylation of the catalytic CDK subunit and inhibition through specific regulatory proteins...