If so, the CTCF binding sites may belong to distinct classes, each compatible with a specific functional role.Kobby Essien¤*Sebastien Vigneau¤Sofia Apreleva¤*Larry N Singh*\u001FMarisa S BartolomeiSridhar Hannenhalli*Essien K, Vigneau S, Apreleva S, Singh LN, Bartolomei MS, Hannenhalli ...
CTCF (CCCTC-binding factor) is an evolutionarily conserved zinc finger protein involved in diverse functions ranging from negative regulation of MYC, to chromatin insulation of the beta-globin gene cluster, to imprinting of the Igf2 locus. The 11 zinc fi
depletion of mutations was observed for constitutive TFBSs (Supplementary Fig.3). This is striking, as two recent studies have found that somatic mutation rates are elevated at transcription factor binding sites (TFBSs), and that this higher overall mutation load at TFBSs may be explained by re...
43], that CTCF heterodimerization with BORIS may lead to more stable DNA binding compared to CTCF homodimerization by itself. To validate whether the reprogrammed set of CTCF binding sites belongs to the clustered CTCF sites class, we conducted a ChIP-Re-ChIP assay and performed motif...
The Insulator Protein CTCF Binding Sites in the orf73/LANA Promoter Region of Herpesvirus Saimiri Are Involved in Conferring Episomal Stability in Latently Infected Human T Cells.pdf 2015-06-16上传 暂无简介 文档格式: .pdf 文档大小: 3.24M ...
17q21 asthma-risk SNPs switch CTCF-binding sites We performed anin silicoanalysis to identify the transcription factors whose binding to thecis-regulatory regions described above is perturbed by asthma-risk SNPs. Our evaluation pointed to perturbation of two CTCF-binding motifs as well as motifs of...
(human, mouse and cow) with the DNA- binding motifs of YY1 and CTCF. This program successfully identified many clustered YY1- and CTCF-binding sites that are conserved among these species but were previously undetected. In particular, this program identified CTCF-binding sites that are located ...
If cell type-specific CTCF binding is of interest, we recommend exploring less significant CTCF sites. To filter the GRanges object and keep high-confidence CTCF sites, use: # Check length before filtering print(paste("Number of CTCF motifs at the default 1e-4 threshold:", length(CTCF_hg38...
Recently, it has been reported that CTCF can be modified by SUMO proteins and the SUMOylation sites identified are at lysine 74 and 698 of the mouse CTCF protein (23). In the present study, we identified that lysine 74 and 689 in human CTCF protein can be modified by SUMO, which is ...
As expected, the recruitment of HP1α to the satellite 2 sites was significantly reduced by vigilin knockdown in HepG2 cells (Fig. 5A). Interestingly, ectopic expression of CTCF weakened the occupancy of HP1α at satellite 2 locus (Fig. 5C). As shown in Fig. 5B and D, the ...