首先，作者通过对发育中的小鼠四肢进行CTCF ChIP-seq，确定在Epha4-Pax3（简称EP）边界区域存在6组CBSs。并且，由CBSs所编码和调控的EP元件具有绝缘性能。边界区域往往主要由CBSs构成，这也提示了此位点数目很可能与其功能相关。作者确定，CBS的数目的确可以影响其绝缘性，但每一个位点的独特特征是决定边界区域生物学功...
If so, the CTCF binding sites may belong to distinct classes, each compatible with a specific functional role. We have classified approximately 26,000 CTCF binding sites in CD4+ T cells into three classes based on their similarity to the well-characterized CTCF DNA-binding motif. We have ...
CTCF (CCCTC-binding factor) is an evolutionarily conserved zinc finger protein involved in diverse functions ranging from negative regulation of MYC, to chromatin insulation of the beta-globin gene cluster, to imprinting of the Igf2 locus. The 11 zinc fi
转录抑制子CCCTC-结合因子(简称CTCF)存在于大部分边界区域之中【4】,其缺失会导致全基因组缺失TADs【8-10】。在TAD边界区域,CTCF结合位点(CTCF binding site,简称CBSs)在脊椎动物进化中高度保守。染色质环的形成通常与TAD边界密切相关,且常常形成于CBSs对之间。这一偏好性可以用“环挤出模型(loop extrusion model)”...
该研究在小鼠中产生了从配子到囊胚过程中CTCF结合位点的动态图谱,发现了一批衍生出卵裂期特有的CTCF结合位点(cleavage-specific CTCF binding sites, cs-CBSs),探究了cs-CBS产生和消失的内在机制,揭示了其在细胞从全能性向多能性转换这一...
CCCTC-binding factor (CTCF) is an architectural protein that can mediate both interchromosomal and intrachromosomal interactions. The functional outcomes of these interactions depend on the nature of the sequences adjacent to CTCF-binding sites and perhaps on the presence of other chromatin proteins. ...
sites into units of active transcription. BORIS binding initiates the recruitment of the chromatin remodeling factor, SRCAP, followed by the replacement of H2A histone with H2A.Z, resulting in a more relaxed chromatin state in the nucleosomes flanking the CTCF binding sites. The relaxation of ...
(insertions/deletions) in three gastric lineage-specific genes. We further identify 34 mutation hotspots, of which 11 overlap CTCF binding sites (CBSs). These CBS hotspots remain significant even after controlling for a genome-wide elevated mutation rate at CBSs. In 3 out of 4 tested CBS ...
5.通过体外R-loop形成实验来证明R-loop确实在CTCF-TAD(CBS)位点处形成,详细步骤为分别构建Cy5标记HOTTIP的R-loop forming region RNA单链(含G-4motif,这个不是G4s),与Cy3标记的CBS的双链DNA,在体外R-loop binding buffer的支持下形成R-loop,通过凝胶迁移实验观察红光与绿光的位置来判断R-loop是否形成,在此基础...
The Insulator Protein CTCF Binding Sites in the orf73/LANA Promoter Region of Herpesvirus Saimiri Are Involved in Conferring Episomal Stability in Latently Infected Human T Cells.pdf 2015-06-16上传 暂无简介 文档格式: .pdf 文档大小: 3.24M ...