When using focused ion beam (FIB) milling under cryogenic conditions, an ion beam is directed onto the surface of the frozen-hydrated sample to precisely remove material. FIB technology can produce thin, electron-transparent cryo-lamellae samples for imaging on a cryo-transmission electron microscope...
Cryo focused ion beam applications FIB microscopes can be used for a range of applications, including sample preparation for transmission electron microscopy as well as subsurface characterization and 3D visualization. Cryo-FIB milling technology Plasma FIB technology Preparing lamellae for cryo-electron...
Cryo-focused ion beam (FIB) milling is emerging as a powerful technique for sample thinning prior to cryotomography imaging. Previous cryo-FIB milling reports utilized custom-built non-standard equipment. Here we present a workflow and the required commercially available instrumentation to either ...
The iFLM Correlative System (Integrative Fluorescence Light Microscope) allows you to combine fluorescence imaging and ion milling within a single cryo-FIB SEM microscope. Explore applications Aquilos Cryo-FIB resources Learn more from scientists who are using the Aquilos Cryo-FIB and take a closer...
Cryo-focused ion beam (cryoFIB) milling4,5 is a promising technique for cryoET sample preparation. In contrast to conventional cryo-ultramicrotomy using a diamond knife6, cryoFIB can avoid mechanical damage to the sample during the sectioning process. However, the ion beam has very low efficien...
Cryo-focused-ion-beam (cryo-FIB) milling has been used to carve (micromachining) out 100–250-nm-thin regions (called lamella) in the intact frozen cells. This procedure opens a window into the cells for high-resolution cryo-ET and structure determination of biomolecules in their native ...
其工作流程是首先将样品(如电镜载网上生长的真核细胞)玻化,然后在扫描电子显微镜(SEM)内使用聚焦离子束(FIB)减薄到适当的厚度(100-300纳米)。最后,用透射电子显微镜(TEM)采集一系列图像。这些二维图像然后用来重建一个三维体积图像,即所谓的断层图。从断层图中,可以重建感兴趣的蛋白质的高分辨率结构模型。在所有...
Cryo-focused ion beam (FIB)-milling of biological samples can be used to generate thin electron-transparent slices from cells grown or deposited on EM grids. These so called cryo-lamellae allow high-resolution structural studies of the natural cellular environment by in situ cryo-electron tomography...
Prior to imaging with cryo-ET, a thin cryo-lamella sample was prepared from the vitrified cell by cryo-focused ion beam (cryo-FIB) milling with the Thermo Scientific Aquilos™ Cryo-FIB. Data segmentation and visualization by Thermo Scientific Amira™ Software. Data courtesy of Dr. Benjamin...
Abstract: Cryo-focused ion beam milling of frozen-hydrated cells has recently provided unprecedented insights into the inner space of cells. In combination with cryo-electron tomography, this method allows access to native structures deep inside cells, enabling structural studies of macromolecules in sit...