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Discussion PCa genetic risk SNPs are enriched in noncoding CREs rather than in protein-coding regions9,80. It is challenging to systematically evaluate the importance of these rCREs in cancer biology and the clinic. Our study demonstrates that CRISPRi mediated loss-of-function screen of rCREs is...
Here, we review key studies that demonstrate the utility of CRISPR screens in deciphering the molecular determinants of sensitivity and tolerance to toxic substances. We reflect on key considerations for implementing a CRISPR screen in toxicology. We also discuss computational methods used for analyzing...
Review doi: 10.3390/ijms222312777. CRISPR Screen Contributes to Novel Target Discovery in Prostate Cancer CRISPR 筛选有助于发现前列腺癌的新靶点 前列腺癌 (PCa) 是男性成人常见的恶性肿瘤之一。组学技术的最新进展,特别是在下一代测序方面,...
CRISPR screening is a large-scale experimental approach used to screen a population of mutant cells to discover genes involved in a specific phenotype. Instead of repressing genes at the post-transcriptional level, CRISPR introduces mutations to genes that render them nonfunctional. ...
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function, but can be confounded by nuclease-induced toxicity at both on- and off-target sites, likely due to DNA ...
Faculty Opinions recommendation of A genome-wide CRISPR-based screen identifies KAT7 as a driver of cellular senescence. T Cai - Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature 被引量: 0发表: 2022年 Csde1 binds transcripts involved in protein homeostasis and controls ...
To perform CRISPR screening using GC-based cell sorting, it is necessary to determine what to screen as the target positive phenotype and train the machine classifier using training samples. The design and preparation of positive and negative phenotypes in the step of training classifiers are the ...
Gene sets in sub-genome screens were defined with FDR < 0.25 threshold separately for enriched and depleted genes, and GSEA enrichment was performed by ranking genes by signed STARS score in the genome screen. Significance was assessed by the GSEA algorithm permutation test. b, Genes ranked...
In vivo genome-wide CRISPR knockout screen in TNBC To start deciphering cancer vulnerabilities and tumor suppressor gene hypersensitivity in TNBC, we performed a pooled genome-wide CRISPR loss-of-function screen using a lentiviral knockout library (GeCKOv2). The library contains 65,383 single guide...