Finally, we discuss current challenges and future directions for using CRISPR-based screens in furthering our understanding of neurological diseases and developing potential therapeutic strategies. Despite challenges, CRISPR-based screens have enormous potential for advancing the therapeutic development of many...
et al. On the design of CRISPR-based single-cell molecular screens. Nat Methods 15, 271 (2018).Hill AJ, McFaline-Figueroa JL, Sarita LM, Gasperini MJ, Matreyek KA, Packer J, et al. On the design of CRISPR-based single cell molecular screens. Biorxiv. 2018;...
Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell 2018, 175:1958–1971 e1915.https://doi.org/10.1016/j.cell.2018.10.024. Geurts MH, Clevers H. CRISPR engineering in organoids for gene repair and disease modelling. Nat Reviews Bioeng. 2023...
Genome-wide bidirectional CRISPR screens identify mucins as host factors modulating SARS-CoV-2 infection - PubMed. https://pubmed.ncbi.nlm.nih.gov/35879412/. Accessed 18 Jan 2024 A genome-wide CRISPR screen identifies UFMylation and TRAMP-like complexes as host factors required for hepatitis A...
Choosing CRISPR-based screens in cancer 来自 Semantic Scholar 喜欢 0 阅读量: 23 作者:Marx,Vivien 摘要: Many possibilities for parsing cancer emerge when labs combine gene editing and screens. And RNAi retains its spot in the menu of options....
转自https://www.youtube.com/watch?v=t3fk560-BTY&t=133s, 视频播放量 120、弹幕量 0、点赞数 4、投硬币枚数 2、收藏人数 7、转发人数 1, 视频作者 辣咖咖, 作者简介 辣辣是星星BIOSTAR,相关视频:4) CRISPR Cas9 - Gene Regulation with dCas9,scalable scCRISPR sc
Tiling CRISPRi screens map gene-specific CREs across the costimulatory locus. Credit:Nature Genetics(2024). DOI: 10.1038/s41588-024-01743-5 Healthy T cells are the foundation of a balanced immune system. They can spring into action to fight off infections or cancer, but keep from becoming so...
The functional domains of different CRISPR effectors and their applications in genome-scale screens. Multiple CRISPR effectors are accessible for disrupting coding and noncoding DNA and RNA segments. One commonly employed CRISPR effector is the CRISPR-associated 9 (Cas9) nuclease, which precisely cuts ...
内容提示: nature biotechnology volume 34 number 6 June 2016 621attest to the high sensitivity of CRISPR-based knockout screens, but differ in their assessment of the relative performance of RNAi screens.RNAi screens in either arrayed or pooled format have been employed to study many different ...
CRISPR screens are particularly powerful in combination with induced pluripotent stem cell technology, which enables the derivation of differentiated cell types, such as neurons and glia, and brain organoids from cells obtained from patients. Modelling of disease-associated changes in gene expression via...