In follow-up work, Chen, Sanjana et al. validated the scoring genes by showing that different guide RNAs targeting these genes enhanced metastasis formation when tested individually in the same in vivo assay that was used in the screen. Moreover, the investigators also formed a focused pool...
TargetingIkzf1(from M3) resulted in the strongest accumulation of intratumoral CTLs (Fig.1b). To explore cell-intrinsic roles ofIkzf1, we used a dual-colour transfer system16,23, wherein the use of different fluorescent proteins did not alter CTL responses (Extended Data Fig.3a). OT-I ...
MIC-Drop will also prove useful in the hands of chemical biologists seeking to identify targets of small molecules that cause phenotypic changes in zebrafish. By using MIC-Drop, a typical screen of 100 genes can be conducted within 2-3 weeks by a single user. This protocol for Multiplexed ...
首先作者通过检索DepMap Avana CRISPR-Cas9 screen dataset 并筛选与AML细胞生长密切相关的基因，随后与Broad Institute 和 Novartis shRNA screens 筛选出来的对AML细胞存活起关键作用的基因取交集，筛选出200个潜在的基因。作者对这200个基因，每个基因设计了3个sgRNA,以及3个靶向到这些基因的内含子区域的sgRNA，另外设...
文献分享 20231104 ZhangWXA genome-wide in vivo CRISPR screen identifies essential regulators of T cell migration to the CNS in a multiple sclerosis model(doi:10.1038/s41593-023-01432-2), 视频播放量 306、弹幕量 0、点赞数 5、投硬币枚数 1、收藏人数 14、转发
2020年7月27日,哈佛大学医学院Joslin糖尿病中心伊鹏和Stephan Kissler合作在Nature Metabolism杂志上发表文章Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes,利用全基因组CRISPR筛选发现RNLS基因突变可保护I型糖尿病中的胰岛细胞不受自身免疫系统识别和攻击...
杂志发表了题为:Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes 的研究论文。 该研究通过体内CRISPR基因组筛选,成功找到了1型糖尿病(T1D)中β细胞的保护性把靶标。抑制RNLS基因可有效保护1型糖尿病(T1D)的β细胞。 该研究还发现了已被FDA批准的...
A pooled in vivo screen using SCAR in a CRISPR-antigen-sensitive renal cell carcinoma revealed resistance pathways associated with autophagy and major histocompatibility complex class I (MHC class I) expression. Thus, SCAR presents a resource that enables CRISPR-based studies of tumor-immune ...
CRISPR-Cas9筛选技术可以应用于药物靶点筛选中,通过大规模筛选技术,可以系统的分析、验证一些与抗药性相关的基因,从而为疾病治疗提供相关数据。美国加州大学圣地亚哥分校的研究人员在国际顶尖学术期刊Nature子刊Nature Cancer期刊上发表的题为“An in vivo genome-wide CRISPR screen identifies the RNA-binding protein Staufe...
CD8 T cells play essential roles in anti-tumor immune responses. Here,we performed genome-scale CRISPR screens in CD8 T cells directly under cancer immunotherapy settings and identified regulators of tumor infiltration and degranulation. The in vivo screen robustly re-identified canonical immunotherapy ...