only some of which drive oncogenesis at certain times during cancer evolution. Identifying the cancer-driver alterations amongst the vast swathes of “passenger” changes still remains a major challenge. Transposon and CRISPR screens in vivo provide complementary methods for achieving this, and each pl...
The immune system can eliminate tumors, but checkpoints enable immune escape. Here, we identify immune evasion mechanisms using genome-scale in vivo CRISPR screens across cancer models treated with immune checkpoint blockade (ICB). We identify immune eva
Notably, USP15 and SCAF1 alterations are observed in 31% of pancreatic cancer patients. Our results highlight the utility of in vivo CRISPR screens to integrate human cancer genomics and mouse modeling for the discovery of cancer driver genes with potential prognostic and therapeutic implications. ...
& Chen, S. Cancer CRISPR screens in vivo. Trends Cancer 4, 349–358 (2018). Article CAS PubMed PubMed Central Google Scholar Wang, X. et al. In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target. Cell 184, 5357–...
While CDK4/6 inhibitors have revolutionized HR breast cancer therapy, there is limited understanding of their efficacy in TNBC and meaningful predictors of response and resistance to these drugs remain scarce. We conducted an in vivo genome-wide CRISPR screen using palbociclib as a selection pressure...
Here, we iteratively develop a multiplex in vivo perturb-seq CRISPRi platform for single-cell genetic screens in cancer and tumor microenvironment cells that leverages intracranial convection enhanced delivery of sgRNA libraries into mouse models of GBM. Our platform enables potent silencing of drivers ...
首先作者通过检索DepMap Avana CRISPR-Cas9 screen dataset 并筛选与AML细胞生长密切相关的基因,随后与Broad Institute 和 Novartis shRNA screens 筛选出来的对AML细胞存活起关键作用的基因取交集,筛选出200个潜在的基因。作者对这200个基因,每个基因设计了3...
为了进一步找出造成细胞可塑性和抗药性的根源,研究人员通过CRISPR library screen筛选的方式敲除了大量可能造成细胞可塑性的基因,结果发现甲基胞嘧啶双加氧酶TET2是造成这些细胞可塑性和抗性的最关键因子。为了进一步确定TET2和其驱动的5hmC变化的...
7. The gene regulatory network that underlies CTL differentiation and whether Texcell responses can be functionally reinvigorated are incompletely understood. Here we systematically mapped causal gene regulatory networks using single-cell CRISPR screens in vivo and discovered checkpoints for CTL ...
Immunotherapy with PD-1 checkpoint blockade is effective in only a minority of patients with cancer, suggesting that additional treatment strategies are needed. Here we use a pooled in vivo genetic screening approach using CRISPR–Cas9 genome editing in