Positive Screen In a positive screen, the goal is to identify those cells that survive post-selection. The selective pressure must be strong enough that most of the cells die, removing their plasmids from the population, and only a small fraction survive. After the surviving cells are collected...
The signal of negative selection is very subtle, but is detectable in several cancer types and in a pan-cancer data set. It is enriched in cell-essential genes identified in a CRISPR screen, as well as in genes with reported roles in cancer.Donate...
Genome-wide CRISPR/Cas9 deletion screen defines mitochondrial gene essentiality and identifies routes for tumour cell viability in hypoxia Article Open access 21 May 2021 RNAi screens identify HES4 as a regulator of redox balance supporting pyrimidine synthesis and tumor growth Article 20 May 2024...
20. Eronia N, Mauri T, Maffezzini E, Bedside selection of positive end-expiratory pressure by electrical impedance tomography in hypoxemic patients: A feasibility study: Ann Intensive Care, 2017; 7(1); 76 21. , ATS/ACCP Statement on cardiopulmonary exercise testing: Am J Respir Crit Care M...
We used CRISPR to screen MCF-7 cells to explore the targets whose inhibition is synthetic lethal with CDK4/6 inhibitors in ER+ breast cancer cells. We found that GATA zinc finger domain containing 1 (GATAD1) is a new synthetic lethal target with CDK4/6 inhibitors in ER+ breast cancer ...
et al. A genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes. Cell 166, 1423–1435 (2016). Article CAS PubMed PubMed Central Google Scholar Ferguson, D. J. & Hutchison, W. M. The host-parasite relationship of Toxoplasma gondii in the brains of chronically ...
(CRHCeA→VTA) and Mor-Ens, which was enhanced after escalating morphine exposure and mediated the negative effect during opiate withdrawal. Pharmacologic intervention or CRISPR-mediated repression of CRHR1 in Mor-Ens weakened the inhibitory CRHCeA→VTAinputs, and alleviated the negative effect during...
AK023948 KO by CRISPR/Cas9. Selection of AK023948 KO clones in MCF-7 cells was carried out using the procedure as described previously22. Briefly, AK023948 dual gRNA and donor vector were co-transfected into the MCF-7 cells in six-well plate using DNAfectin (Applied Biological Materials)...
Containing 123,411 sgRNAs tar- geting 20,914 human genes, the GeCKOv2.0 human library is used as a functional profiling pool for CRISPR/Cas9 genetic selection, which led to the discovery of a series of genes involved in anti-HER2 resistance. Among these candidate genes and related proteins,...
To screen for HIFAL interacting proteins, we employed RNA pull-down assays fol- lowed by mass spectrometry analysis (Fig. 2a). Among all the proteins that were pulled down by HIFAL, PKM2, and PHD3 aroused our interest since they are major components in the HIF- 1α transcriptional complex...