WT’作为一种内参突变可以过滤掉包括脱靶效应在内的大多数影响因素。 图1 CRISPR-Select工作原理示意图 在这项研究中,研究人员首先利用CRISPR-SelectTIME,通过测定不同时间点Variant/WT’的比例变化:将VUS根据功能分类为良性或者致病性,并获...
Phenotypically, CRISPR-Select can thereby determine, for example, pathogenicity, drug responsiveness/resistance or in vivo tumor promotion by a specific variant. Mechanistically, CRISPR-Select can dissect how the variant elicits the phenotype by causally linking the variant to motility/invasiveness or ...
WT’作为一种内参突变可以过滤掉包括脱靶效应在内的大多数影响因素。 图1 CRISPR-Select工作原理示意图 在这项研究中,研究人员首先利用CRISPR-SelectTIME,通过测定不同时间点Variant/WT’的比例变化:将VUS根据功能分类为良性或者致病性,并获得致病性突变的效应强度;还对“驱动”突变进行了研究,发现如果对癌细胞的“驱动...
A HIGH-THROUGHPUT PLATFORM TO SELECT FOR REGULATORS OF CRISPR-CAS ASSOCIATED ACTIVITYThe invention relates to a method for in vivo detection of small molecules or polypeptides that act as regulators of CRISPR-Cas associated activity, in particular the method is for use in the screening of molecule...
P62.02 Identification of Essential Genes in Malignant Pleural Mesothelioma Using Genome-Wide CRISPR/Cas9-Based Negative Selection Screendoi:10.1016/j.jtho.2021.01.976E. CakirogluDokuz Eylul University, Izmir International Biomedicine and Genome Institute, Izmir/TRO. Coskun...