2017. CRISPR-Cpf1: A New Tool for Plant Genome Editing. Trends in Plant Science. V.22, Is. 7; 550 553Zaidi S S,Mahfouz M M,Mansoor S.CRISPR-Cpf1:A new tool for plant genome editing[J].Trends in Plant Science,2017,22(7):550-553....
(1)CRISPR-plant 功能:A Portal of CRISPR-Cas9 Mediated Genome Editing 网址:https://www.genome.arizona.edu/crispr/CRISPRsearch.html 设计高度特异性gRNA的原因:gRNA-Cas9也能识别不完全匹配gRNA间隔区的PAM位点,导致基因组编辑中的脱靶风险。 使用方法:选择search-select species-输入基因的loc号码。 选择标准:...
Genome editingOff-targetPlantThe CRISPR/Cas technology has recently become the tool of choice for targeted genome modification in plants and beyond. Although... Florian,Hahn,Vladimir,... - 《Plant Cell Reports》 被引量: 1发表: 2018年 Bioinformatic Tools in CRISPR/Cas Platform CRISPR/Cas has em...
CRISPR-Cpf1: A New Tool for Plant Genome Editing Clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated proteins (CRISPR-Cas), a groundbreaking genome-engineering tool, has facilit... SEA Zaidi,MM Mahfouz,S Mansoor - 《Trends in Plant Science》 被引量: 21发表: 2017年 DN...
CRISPR is becoming an indispensable tool in biological research. Once known as the bacterial immune system against invading viruses, the programmable capacity of the Cas9 enzyme is now revolutionizing diverse fields of medical research, biotechnology, an
editing has been successfully applied in the genome modification of diverse plant species, such as Arabidopsis thaliana, Oryza sativa, and Nicotiana tabacum... Z Xiangchun,X Yongzhong - Yi chuan = Hereditas 被引量: 7发表: 2016年 基于CRISPR/Cas9技术的烟草NtDXR基因敲除及功能分析 CRISPR/Cas9-mediat...
PAM-less plant genome editing using a CRISPR–SpRY toolbox 原文链接:https://mp.weixin.qq.com/s/jQRc4qIi5V-Y0JY8IcHBhA 买转录组+蛋白质定量项目,送转录组与蛋白质组关联分析!立即咨询>> 立即索取Amnis量化成像流式细胞仪的技术资料和应用文献>> ...
2024年1月18日,Plant Communications在线发表了北京大学现代农学院刘启昆课题组、北京大学现代农业研究院周军会课题组、中国科学院遗传与发育生物学研究所曹晓风课题组的题为“An Inducible CRISPR-activation tool for accelerated plant regeneration”的研究成果。该研究通过对植物内源的组织再生相关基因的精准激活,实现了对...
Fig. 1. Depiction of viral genome editing in plant cell nucleus through CRISPR/Cas9 tool (1) Plant chromosome possessing multiple gRNAs and Cas9 gene. (2) Synthesis of Cas9 protein and transcription of gRNAs to pre-crRNA. (3) Formation of cr-RNA and Cas9 complex. (4) Viral genome entry...
New and powerful way to edit genomes and control gene function based on a talk about the TAL effector proteins that were first identified as bacterial plant pathogens. 支持基因编辑方法和应用的特色产品 Invitrogen TrueCut Cas9 蛋白 Invitrogen TrueTag Donor DNA ...