The present invention relates to optimized systems for the genome editing of eukaryotic cells, preferably plant cells. Also provided is a plant delivery system comprising at least one Cpf1 enzyme, or a plant optimized construct encoding the same, to be synergistically combined with a Cpf1 guide ...
植物可通过调节气孔开闭和气孔密度调节水分利用效率,从而调控植物抗旱能力。 近日,中国农业大学园艺学院郭仰东课题组在Plant, Cell & Environment(IF:6.362)在线发表了题为SlTLFP8 reduceswater loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication的研究论文。该研究...
Since the beginning of CRISPR/Cas9's use for genome-editing, reports of off-targets have caused concerns among users. Previous studies identified three main types of off-targets. The first type of off-targets are sequences at other 5'-NGG-3' protospacer adjacent motifs (PAMs) that are ident...
Heritability of targeted gene modifications induced by plant- optimized CRISPR systems. Cellular and Molecular Life Sciences:1-19.Mao Y F,Botella J R,Zhu J K.Heritability of targeted gene modifications induced by plant-optimized CRISPR systems[J].Cellular and Molecular Life Sciences,2017,74(6):...
近日,江苏里下河地区农业科学研究所的研究团队在《Plant Communications》在线发表了题为“CRISPR/Cas9-mediated editing ofOsHPPD3’-UTR confers enhanced resistance to HPPD-inhibiting herbicide in rice”的研究论文,介绍了研究团队利用CRISPR/Cas9和CRISPR/Cas12a基因编辑系统对水稻HPPD抑制剂抗性基因OsHPPD的3’-UT...
CRISPR因组编辑技术大规模构建突变体库并进行功能筛选是高效便捷获得重要突变体和快速克隆对应基因的有效方法,同时能够为分子设计育种提供重要的供体材料。虽然该技术具有巨大的应用潜力,但在植物中的应用仍处于起步阶段。近日,The Plant Cell 在线发表了比利时VIB-UGent植物系统生物学中心的Thomas B Jacobs教授的题为CRISP...
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe th...
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe th...
The potential for clustered regularly interspaced short palindromic repeats (CRISPR)-related applications in prokaryotes can be used for microorganisms' identification and their quantitation such as pathogens and food spoilage bacteria and modulate the composition of bacterial communities for a variety of ...
然而,在植物中进行基因编辑时,不仅受限于植物组织培养或转化技术,而且T-DNA随机整合到编辑植物的基因组中也带来了转基因监管和生物安全问题。因此,各种植物病毒已经被设计成可以容纳和传递CRISPR/Cas的微型载体来产生无转基因和基因组编辑的植物。 2023年5月24日,国际著名杂志Trends in Plant Science杂志在线发表了题...