Genetic variance is vital for breeding programs and mutant screening, yet traditional mutagenesis methods wrestle with genetic redundancy and a lack of specificity in gene targeting. Here the authors develop a scalable CRISPR system in tomato to edit multiple genes at once, overcoming genetic redundancy...
Sharing of whole genome gRNA library data, pooled libraries, and even complete transformed CRISPR mutant populations in the form of seeds could make a usage boost to functional studies. As mentioned above, 4,604 nuclear plant genomes have been sequenced, corresponding to 1482 plant species (Fig....
Application of protoplast technology to CRISPR/Cas9 mutagenesis:From single cell mutation detection to mutant plant regeneration. Lin C S,Hsu C T,Yang L H,et al. Plant Biotechnology Journal . 2017Lin, C.S.; Hsu, C.T.; Yang, L.H.; Lee, L.Y.; Fu, J.Y.; Cheng, Q.W.; Wu, F...
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for genetic manipulation has revolutionized life science over the past few years. CRISPR was first discovered as an adaptive immune system in bacteria and archaea, and
In plants, the CRISPR systems associated with the Cas9 and Cpf1 nucleases are the best studied and characterized. The editing of plant genomes has been broadly classified into three types: Site Directed Nuclease (SDN)1, 2 and 3. SDN1 is repair of CRISPR generated DSBs through the NHEJ pathw...
(TALEN), the authors induced local mutations into the polyploid genome of two potato cultivars. Many researchers emphasize the importance of using TALEN as a system capable of generating a less off-target rate than the conventional CRISPR-Cas approach. This is of particular importance for ...
Post-genomic era of CRISPR/Cas technology in ornamental plants: advantages, limitations, and prospects Ambreena Din, Muneeb Ahmad Wani, Chunlian Jin, Imtiyaz Tahir Nazki, Junren Ma & Fan Li 2025, 5: e010, doi: 10.48130/opr-0025-0007 REVIEW 07 Mar 2025Open Access GC-MS metabolite an...
Gene-editing tools, such as CRISPR/Cas9, enable precise manipulation of plant metabolic pathways, facilitating the synthesis of antiviral compounds. For instance, in soybean, CRISPR/Cas9-mediated multiplex gene editing targeted three key genes involved in flavonoid biosynthesis—GmF3H1, GmF3H2 and ...
为了进一步研究BnaZEPs的功能,构建了2个聚集的规则间隔短回文重复序列(CRISPR)/CRISPR相关9(Cas9)载体,并将其转化为黄色的甘蓝型油菜。一个载体靶向BnaA07.ZEP和BnaC07.ZEP,而另一个靶向所有4个BnaZEP。两个纯合T2双突变体(Bnaa07c07zep)和两个四重突变体(Bnatzep)用于随后的分析。在这些品系中,所有的靶等位...
(e.g., to avoid a yield penalty), it would be possible to make a stable knock-down mutant with the specificity of CRISPR/Cas9, as is recently demonstrated forccrin poplar [28]. In addition, finally, (iii) by cr...