The application of clustered regularly interspaced short palindromic repeats (CRISPR) for genetic manipulation has revolutionized life science over the past few years. CRISPR was first discovered as an adaptive immune system in bacteria and archaea, and
1d). Each of the mutant lines harbors a particular mutation that leads to a CCD7 protein variant (Fig. 1e, Additional file 1: Figure S2). These protein variants produce variation in tillering and plant height (Fig. 1d). One advantage of CRISPR/Cas9 mutagenesis is that the mutation can ...
Tissue culture, somatic embryogenesis, genetic transformation and somatic hybridization are essential for cultivar improvement; Preservation and cryopreservation are ideal means for passion fruit long time preservation; This review provides biotechnological tools and a current understanding of Passiflora spp. Pro...
Open Access Collection Issue Information 2023Plant Biotechnology Journal doi:10.1111/pbi.13854 journal article Download Only Collection Creation of herbicide‐resistance in allotetraploid peanut using CRISPR/Cas9‐meditated cytosine base‐editing Shi, Lei;Li, Xiaona;Xue, Lulu;Zhang, Jin;Huang, Bingyan;Sun...
Further functional analysis indicated that these ASIGs may make important contributions to kernel development. Knockout mutant of one ASIG influence the length and width of the kernel. This study provides valuable resources for the konwledge of ASIGs in maize or even cereals....
CRISPR base editing inS. iranensis To validate the in silico prediction, we utilised the efficient base editing tool CRISPR-cBEST to experimentally confirm theptaBGC54. As a non-modelStreptomycesstrain,S. iranensisis hard to genetically manipulate through intergeneric conjugation55. Therefore, the con...
Yan et al. develop a “pNC” vector system using standardized Nimble Cloning. This system is well-suited for investigating gene ectopic expression, protein subcellular localization, protein–protein interaction, gene silencing (RNAi), virus-induced gene silencing, promoter activity, and CRISPR–Cas9-me...
WOX13A regulates the expression ofGn1and affects maize architecture.AWOX13Aexpression across different tissues and stages.BStrategy for the CRISPR–Cas9-based isolation of aWOX13Aknockout mutant.CDifferential expression analysis based on RNA-seq of V6-stage internodes from wild type andwox13amutant. ...
10d,e). Null mutants of WRKY30 and WRKY28 have not been reported, and our attempts to knock out these TFs using CRISPR failed, suggesting that these genes are essential for plant viability39,40. We therefore focused on MYB15, a positive regulator of defence against the foliar pathogen Pto...
There are many open questions about the mechanisms that coordinate the dynamic, multicellular behaviors required for organogenesis. Synthetic circuits that can record in vivo signaling networks have been critical in elucidating animal development. Here,