CRISPR-PLANT v2: An online resource for highly specific guide RNA spacers based on improved off-target analysisGenome editingCRISPR〤as9guide RNAoff‐target predictionSince the beginning of CRISPR/Cas9's use for genome-editing, reports of off-targets have caused concerns among users. Previous ...
2022年8月3日,韩国庆尚大学植物分子生物学和生物技术研究中心Jae-Yean Kim课题组在Plant Communications在线发表了题为Engineering drought and salinity tolerance traits in crops through CRISPR-mediated genome editing: Targets, tools, ch...
近日,学校生命科学学院张勇教授团队联合园艺园林学院宋洪元教授团队、马里兰大学Yiping Qi教授在Plant Communications上发表了题为Boosting genome editing in plants with single transcript unit surrogate reporter systems的研究论文。该研究工作基于...
The potential for clustered regularly interspaced short palindromic repeats (CRISPR)-related applications in prokaryotes can be used for microorganisms' identification and their quantitation such as pathogens and food spoilage bacteria and modulate the composition of bacterial communities for a variety of ...
- 《Plant Methods》 被引量: 471发表: 2013年 The CRISPR-Cas system for plant genome editing: Advances and opportunities Abstract Genome editing is an approach in which a specific target DNA sequence of the genome is altered by adding, removing, or replacing DNA bases. Artifi... V Kumar,M ...
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe th...
近日,中国农业大学园艺学院郭仰东课题组在Plant, Cell & Environment(IF:6.362)在线发表了题为SlTLFP8 reduceswater loss to improve water‐use efficiency by modulating cell size and stomatal density via endoreduplication的研究论文。该研究发现番茄TLFP8基因通过核内再复制,调节气孔大小和密度,从而减少水分流失,提...
CRISPR因组编辑技术大规模构建突变体库并进行功能筛选是高效便捷获得重要突变体和快速克隆对应基因的有效方法,同时能够为分子设计育种提供重要的供体材料。虽然该技术具有巨大的应用潜力,但在植物中的应用仍处于起步阶段。近日,The Plant Cell 在线发表了比利时VIB-UGent植物系统生物学中心的Thomas B Jacobs教授的题为CRISP...
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe th...
2021年4月5日,根特大学Thomas B Jacobs研究团队在The Plant Cell发表了题为CRISPR Screens in Plants: Approaches, Guidelines, and Future Prospects的综述文章,文章对CRISPR筛选技术在植物中的应用进行了综述:植物CRISPR筛选技术的发展为更高分辨率和更大规模地分析植物基因组提供了新的机会,并将极大地促进功能生物学...