On the basis of the analyses carried out on the two possible futures for CRISPR-edited plants and of their potential with respect to plant breeding, the main conclusions are the following. Firstly, that the regulatory review that started in 2021 is not in itself "good ...
Thus, some methods of plant transformation that do not depend on in聽vitro regeneration have been developed. These methods are known as " in planta " transformation. The main focus of this review is the so-called floral dip in planta transformation method, although other approaches are also ...
Targeted modification of plant genome is key to elucidating and manipulating gene functions in plant research and biotechnology. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) techn... Jian-Feng Li *,Dandan Zhang *,Jen Sheen * - 《Methods in ...
近日,nature plants在线发表了美国马里兰大学Yiping Qi团队题为“The emerging and uncultivated potential of CRISPR technology in plant science”的综述性文章,系统的综述了CRISPR技术在植物科学领域的应用和挑战。 The application of clustered regularly interspaced short palindromic repeats (CRISPR) for genetic manipu...
However, challenges in plant genome editing need to be fully appreciated and solutions explored. This Review intends to provide an informative summary of the latest developments and breakthroughs of CRISPR technology, with a focus on achievements and potential utility in plant biology. Ultimately, ...
CRISPR-Cpf1: A New Tool for Plant Genome Editing Clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated proteins (CRISPR-Cas), a groundbreaking genome-engineering tool, has facilit... SEA Zaidi,MM Mahfouz,S Mansoor - 《Trends in Plant Science》 被引量: 21发表: 2017年 DN...
“It takes plant breeding to the next level,” Doudna says of CRISPR. “We can adjust the numbers of those pores by dialing up or down certain genes … to the levels that actually support plant growth [and] allow farmers to produce rice of the quality and with t...
There were several CRISPR/CRISPR-associated gene (Cas)-based methods studied in the current review, taking into account the recent progress and challenges involving those against pathogenic bacteria in crop improvement programs. This chapter also discusses how using this integrated approach permits ...
plant synthetic biology. In this Review, we first briefly describe gene editing by CRISPR–Cas, focusing on the newest, precise gene editing technologies such as base editing and prime editing. We then discuss the most important applications of CRISPR–Cas in increasing plant yield, quality, ...
Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of recombinant Cpf1 proteins with in vitro transcribed or chemically synthesized target-specific crRNAs into protoplasts isolated from soybean and wild tobacco. Designed crRNAs are ...