With a goal of breeding resilient crops that are better able to withstand drought and disease, University of California San Diego scientists have developed the first CRISPR-Cas9-based gene drive in plants. The research, led ...
Moreover, we develop a potent near-PAM-less CRISPR–Act3.0 system on the basis of the SpRY dCas9 variant, which outperforms the dCas9–NG system in both activation potency and targeting scope. Altogether, our study has substantially improved the CRISPRa technology in plants and provided plant...
Results: In this study, we investigate whether the CRISPR/Cas9 system could be used in plants to confer molecular immunity against DNA viruses. We deliver sgRNAs specific for coding and non-coding sequences of tomato yellow leaf curl virus (TYLCV) into Nicotiana benthamiana plants stably ...
No.2 表 1 本研究中所用的引物 Table 1 Primers used in this study 用途 Purpose 合成靶位点序列 Synthetic target site 潮霉素抗性基因检测 Hygromycin resistance gene detection Cas9 基因检测 Cas9 gene detection 检测转基因突变 Detection of mutations in transgenic plants 引物名称 Primer name BoZDS-CRISPR...
The T0 generation ZmFKF1 gene editing positive plants were obtained and verified by using the specific primers of cas9. The target site amplification and sequencing analysis was used to determine whether the T1 transgenic lines had mutation at the expected target site of ZmFKF1 and the type of ...
Currently, a number of cases have been reported on the application of the CRISPR/Cas9 genomic editing technology in plants. In this review, we summarize the strategies for preparing the Cas9 and sg RNA expression constructs, the transformation method for obtaining targeted mutations, the efficiency ...
Toda, E., Koiso, N., Takebayashi, A., Ichikawa, M., Kiba, T., Osakabe, K., Osakabe, Y., Sakakibara, H., Kato, N. and Okamoto, T. (2019) An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice. Nature Plants, 5, 363-368. ...
A patent application was filed on CRISPR/Cas9-mediated virus interference in plants. Authors’ contributions MMM and ZA designed the study; ZA, AA, AI, SA, and MT performed the research; MMM and ZA analyzed the data; MMM wrote the manuscript. All authors read and approved the final ...
plants ofArabidopsisand rice, respectively.(EandF)Representative T1 transgenic plants of theBRI1sgRNA1 target. The left one is a T1 plant with normal vegetative growth, while the right one is a plant with a similar growth phenotype asbri1mutants. The plants were screened on MS plates for 5...
Despite its popularity, the technology has limitations such as off‐targets, low mutagenesis efficiency, and its dependency on in‐vitro regeneration protocols for the recovery of stable plant lines. Several other issues such as persisted CRISPR activity in subsequent generations, the potential for ...