M. Connolly et al., "CpG-free plasmid expression cassettes for cystic fibrosis gene therapy," Biomaterials, vol. 33, no. 28, pp. 6833-6842, 2012.Pringle IA, Hyde SC, Connolly MM, et al. CpG-free plasmid expression cassettes for cystic fibrosis gene therapy. Biomaterials 2012;33:6833-42...
Review of the effect of CpG dinucleotide on the immune response and the gene silencing of gene delivery vectors for gene therapy applications.
Using a CpG-free pDNA expression vector, we achieved sustained (>or=56 d) in vivo transgene expression in the absence of lung inflammation.关键词: elf`18 fl-22 flagellin PARG Psetidomonas syringae pv. glycinea P. syringae pv. tomato ...
pCpGfree is a family of plasmids completely devoid of CpG dinucleotides. Typically, the elements required for replication and selection of the plasmid in E. coli and gene expression in mammalian cells are rich in CpG. In the pCpGfree plasmids these eleme
To determine whether ITR modification is a viable approach for AAV vector engineering, we rationally deleted all CpG motifs in the ITR and examined whether CpG elimination compromises AAV-vector production and transduction. Modified ITRs were stable in the plasmid and maintained the CpG-free nature ...
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CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat Biotechnol 2008; 26: 549–551. Article CAS Google Scholar Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A . Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol...
Lehn, T. Montier, Efficient in vivo transfection and safety profile of a CpG-free and codon optimized luciferase plasmid using a cationic lipophosphorami... Lindberg,F Mattias,G Le,... 被引量: 0发表: 0年 CpG DNA induces sustained IL-12 expression in vivo and resistance to Listeria monocyt...
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