As expected CpG-free DNA (pCpGfree-mcs and genomic calf DNA) induced negligeable amounts of TNF-α whereas CpG-rich DNA (pDNA-CMV, CpG ODN1826 andE. coli DNA) induced significant amounts of TNF-α. All DNAs injected were endotoxin-free. ...
mEPO expression and hematologic levels were significantly lower when using comparable amounts of the wild type (mEPOwt) gene and only marginal effects were induced by mEPOCpG lacking intragenic CpG dinucleotides, even at high pDNA amounts. Corresponding with these observations, in vitro analysis of...
Resistance to the antibiotic Zeocin™ is conferred by the Sh ble gene from Streptoalloteichus hindustanus. The DNA sequence of this gene was modified by optimizing the codon usage and deleting 50 CpG motifs without changing the amino acid sequence of t
Thus, a novel SS DNA vaccine-fused CpG motifs should be constructed, and its effects in promoting the growth of animals should be evaluated to improve the efficiency and reduce the cost of SS DNA vaccine. Evaluation of the Fusion Type CpG Adjuvant for the Enhancement of Somatostatin DNA Vaccin...
Advanced gene delivery techniques can be combined with rational gene design to further improve the efficiency of plasmid DNA (pDNA)-mediated transgene expression in vivo. Herein, we analyzed the influence of intragenic sequence modifications on transgene expression in vitro and in vivo using murine er...
It is unclear whether DNA methylation of these circadian E-boxes and the surrounding CpG islands would affect protein/DNA interactions and lead to distinct binding features. In the present study, bisulfite sequencing analysis indicated that all E-boxes and their surrounding CpG islands were free ...
CpG DNA enhances the im-mune responses elicited by the DNA vaccine against foot-and-mouth disease virus in guinea pigs Our study demonstrated that it is an effective route to enhance the efficacy of DNA vaccines by inserting exogenous CpG DNA into the plasmids, and the ... LI Guangjin,LI ...
Objective To study the DNA methylation of E-boxes which may or may not be involved in the protein/DNA interacting and in the MKP1 tissue-specific expressions. To study the DNA methylation of E-boxes which may or not involved in the protein/DNA interacting and in the MKP1 tissue-specific ...
United States Patent US8017749 Note: If you have problems viewing the PDF, please make sure you have the latest version ofAdobe Acrobat. Back to full text
Determining the degree of methylation of a target cytosine in the genome of a cell or organism comprises treating a DNA sample with bisulfite, amplifying a fragment of the DNA, sequencing the amplified fragment to generate an electropherogram, determining the rate of conversion of cytosine to ...