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Recently, CpGs in plasmid DNA (pDNA) were found to enhance the clearance of PEG-coated pDNA-lipoplexes, a phenomenon that could be reduced by the use of CpG-free pDNA allowing repeated dosing[5]. DNA-induced immune response.Mice were injected i.p. with 500 μg CpG-rich or CpG-free...
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat. Biotechnol. 2008; 26 :549–551. doi: 10.1038/nbt1399. [ Cross Ref ]Hyde SC, Pringle IA, Abdullah S et al (2008) CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. ...
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In the pCpGfree plasmids these elements are either naturally CpG-free, were modified to remove all CpGs, or entirely synthesized. Back to the top Specifications pCpGfree-mcs contains a multiple cloning site (MCS) featuring several commonly used restriction sites, for convenient cloning of a CpG...
To study the biological potency, we made micro-dystrophin (μDys) AAV vectors carrying either the wild-type ITR or the CpG-free ITR. We delivered the CpG-free μDys vector to one side of the tibialis anterior muscle of dystrophin-null mdx mice and the wild-type μDys vector to the ...
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CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat Biotechnol 2008; 26: 549–551. Article CAS Google Scholar Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A . Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol...
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Expression vector CpG-free for enhancer CpG methylation study pCpGfree-promoter is a reporter plasmid completely devoid of CpG dinucleotides. This plasmid contains a CpG-free allele of a reporter gene (murine SEAP or secreted luciferase Lucia), the human EF-1α promoter and a multiple cloning...