CpG free plasmidSkinImagingSkin is a very suitable target for gene therapy and DNA vaccination due to its accessibility, its surface and its ability to produce transgenes. Gene electrotransfer (GET) to the skin is under development for clinical applications for DNA vaccine or local treatment such...
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammat...
pCpGfree-mSEAP A mSEAP expression plasmid completely devoid of CpG dinucleotides Add to favorite 20 µg pcpgf-mseap TDSMSDSSEQ ￥8,160 Please contactour distributor pCpGfree-LacZ A LacZ expression plasmid completely devoid of CpG dinucleotides ...
CpG-free plasmid backbone Reporter plasmid without an enhancer/promoter region: murinesecretedalkaline phosphatase (SEAP) secreted luciferase (Lucia) Selectable inE. coliwith Zeocin™ This product is covered by a Limited Use License (seeTerms and Conditions). ...
Modified ITRs were stable in the plasmid and maintained the CpG-free nature in purified vectors. Replacing the wild-type ITR with the CpG-free ITR did not affect vector genome encapsidation. However, the vector yield was decreased by approximately 3-fold due to reduced vector genome replication....
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat Biotechnol 2008; 26: 549–551. Article CAS Google Scholar Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A . Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol...
产品名称: pSELECT-zeo-hSEAP-CpG-free .0 订购此产品 供应商: Invivogen 规格: 20 μg 目录价: 5698 库存状态: 三周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: CpG-free SEAP Synthetic SEAP reporter gene in expression plasmid InvivoGen provides an optimized SEAP reporter gene ...
Whereas pTG11033 plasmid is driven by a strong ubiquitous cytomegalovirus promoter, pGM144 plasmid, which has been designed to avoid inflammation and provide sustained transgene expression in lungs, is CpG-free and is under control of the human elongation factor-1 alpha promoter. Combined to the ...
Recently, CpGs in plasmid DNA (pDNA) were found to enhance the clearance of PEG-coated pDNA-lipoplexes, a phenomenon that could be reduced by the use of CpG-free pDNA allowing repeated dosing[5]. DNA-induced immune response.Mice were injected i.p. with 500 μg CpG-rich or CpG-free...
20020042383 METHYLATION AND/Or CpG REMOVAL OF PLASMID VECTORS 2002-04-11 Yew et al. 20020028784 Methods of preventing and treating viral infections using immunomodulatory polynucleotide sequences 2002-03-07 Van Nest 20020006403 CD28-specific antibody compositions for use in methods of immunosuppression ...