The pCpGfree-promoter plasmid contains the CpG-free version of the human EF-1α promoter and a multiple cloning site. The synthetic mSEAP∆CpG gene: a CpG-free allele of the murine SEAP gene constructed by chemical synthesis. Polyadenylation signal: The polyadenylation signal is a CpG-fre...
Recently, CpGs in plasmid DNA (pDNA) were found to enhance the clearance of PEG-coated pDNA-lipoplexes, a phenomenon that could be reduced by the use of CpG-free pDNA allowing repeated dosing[5]. DNA-induced immune response.Mice were injected i.p. with 500 μg CpG-rich or CpG-free...
Pulmonary delivery of plasmid DNA (pDNA)/cationic liposome complexes is associated with an acute unmethylated CG dinucleotide (CpG)-mediated inflammatory response and brief duration of transgene expression. We demonstrate that retention of even a single CpG in pDNA is sufficient to elicit an inflammat...
CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat Biotechnol 2008; 26: 549–551. Article CAS Google Scholar Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A . Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol...
Modified ITRs were stable in the plasmid and maintained the CpG-free nature in purified vectors. Replacing the wild-type ITR with the CpG-free ITR did not affect vector genome encapsidation. However, the vector yield was decreased by approximately 3-fold due to reduced vector genome replication....
产品名称: pSELECT-zeo-mSEAP-CpG-free .0 订购此产品 供应商: Invivogen 规格: 20 μg 目录价: 5698 库存状态: 三周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: CpG-free SEAP Synthetic SEAP reporter gene in expression plasmid InvivoGen provides an optimized SEAP reporter gene ...
Phosphorothioate CpG ODN 1555 (GCTAGACGTTAGCGT) and ODN lacking a CpG motif ODN 1556 were synthesized at the CBER core facility. DMEM, phenol red free EMEM, 2X EMEM, penicillin streptomycin solution (100×), L-glutamine solution (100×), trypsin (0.25%)-EDTA(1 mM) and HEPES were purc...
Stable cell lines were engineered using CRISPR/Cas9-mediated genome editing. sgRNAs were designed using the CRISPOR online tool (http://crispor.tefor.net; Supplementary Table1) and oligonucleotides encoding sgRNAs were cloned into the pSpCas9 (BB)-2A-Puro plasmid as previously described (Addgene...
CpG-free plasmid backbone Reporter plasmid without an enhancer/promoter region: murinesecretedalkaline phosphatase (SEAP) secreted luciferase (Lucia) Selectable inE. coliwith Zeocin™ This product is covered by a Limited Use License (seeTerms and Conditions). ...
pCpGfree-mSEAP A mSEAP expression plasmid completely devoid of CpG dinucleotides Add to favorite 20 µg pcpgf-mseap TDSMSDSSEQ ￥8,160 Please contactour distributor pCpGfree-LacZ A LacZ expression plasmid completely devoid of CpG dinucleotides ...