Intrauterine infection with plasmid-free Chlamydia muridarum reveals a critical role of the plasmid in chlamydial ascension and establishes a model for evaluating plasmid-independent pathogenicity. Infect Immun. 2015;83(6):2583–92. Article CAS PubMed Google Scholar Song L, Carlson JH, Whitmire WM...
EDTA-free (Thermo Fisher Scientific, Waltham, MA, USA). After sonication and centrifugation at 10,000×gfor 15 min, supernatant proteins (0.4 mg/mL, 150–200×g) were reduced with 25 mM tris(2-carboxyethyl)phosphine for 1 h at room temperature (RT), and free cysteine residues were alkyl...
Article Electrotransfer of Plasmid DNA Encoding an Anti-Mouse Endoglin (CD105) shRNA to B16 Melanoma Tumors with Low and High Metastatic Potential Results in Pronounced Anti-Tumor Effects Tanja Dolinsek 1, Gregor Sersa 1, Lara Prosen 1, Masa Bosnjak 1, Monika Stimac 1, Urska Razborsek 1 ...
ADP was observed to bind four of the six AD–AD interfaces, with the interfaces for the two ADs near the bottom of the staircase being nucleotide free. We were unable to resolve density associated with the six ED, presumably due to their flexibility. Furthermore, we demonstrated that PCV2 ...