copper‐free click chemistryphosphine and azide Staudinger ligationbio‐orthogonal ligationsCu‐catalyzed azide–alkyne cycloadditionketone–aldehyde condensation reactions‘traceless’ Staudinger ligationazide and triarylphosphine Staudinger ligationHoveyda–Grubbs second‐generation catalyst...
■ Copper-free Click Chemistry (DBCO & BCN reagents) Conventional "Click Chemistry" requires the presence of a Cu(I) catalyst that is toxic to most organisms and thus, prevents its use in many biological systems. The novel Copper-free Click Chemistry is based on the reaction of a diary...
Copper-free сlick chemistry is a set of modern orthogonal bioconjugation reactions which take place in the absence of transition metals (like copper). The reactions include strain-promoted alkyne-azide cycloaddition (SPAAC), and tetrazine - trans-cycloo
Click chemistry obviates the requirement of phosphines, but the Cu(I) catalyst is toxic to cells, thereby precluding in vivo applications. Here we ... NJ Agard,JA Prescher,CR Bertozzi - 《Journal of the American Chemical Society》 被引量: 1700发表: 2004年 Profiling enzyme activities in vivo...
Experience copper-free 'click chemistry' with Click-IT™ Alexa Fluor™, and sDIBO Alkyne reagents, which are ideal for surface labeling of live cells and minimize damage to enzymes and fluorescent proteins.Have Questions? 联系我们 Change view Catalog NumberLabel or DyeReactive MoietyMolecular Wei...
International Journal of Molecular Sciences Article Copper-Free 'Click' Chemistry-Based Synthesis and Characterization of Carbonic Anhydrase-IX Anchored Albumin-Paclitaxel Nanoparticles for Targeting Tumor Hypoxia Katyayani Tatiparti 1,†, Samaresh Sau 1,*,† ID , Kaustubh A. Gawde 1 and Arun K. ...
Berkeley Lab researchers have found a way to make copper-catalyzed click chemistry biocompatible. By adding a ligand that minimizes the toxicity of copper but still allows it to catalyze the click chemistry reaction, the researchers can safely use their
Click chemistry: Copper clusters catalyse the cycloaddition of azides with terminal alkynesLD Pachón, JH Van Maarseveen… Index: Advanced Synthesis and Catalysis, , vol. 347, # 6 p. 811 - 815 Full Text: HTMLCitation Number: 228 Abstract... aliphatic azides.[2] Sharpless and co-workers ...
Click-iT sDIBO Alkyne labels react with azides via a copper-free Click chemistry reaction to form a stable triazole linkage with an Alexa Fluor, Biotin, or label of your choice. Copper-free click reactions using our sDIBO alkynes are ideal for surface labeling of live cells; they also minim...
Organic chemistry Development of copper-free click chemistry and application to imaging glycans in living systems UNIVERSITY OF CALIFORNIABERKELEY Carolyn R. Bertozzi BaskinJeremy MichaelImaging biomolecules within living systems requires a means to distinguish the target from the surrounding components using ...