The invention provides a construction method of a stable cell line realizing FTH1 gene controlled expression. The construction method comprises the following steps: (1) acquiring FTH1 genes; (2) transferring the FTH1 genes into a Tet-On vector to obtain recombinant plasmids; (3) constructing ...
Lentiviral vectors are useful experimental tools for stable gene delivery and have been used to treat human inherited genetic disorders and hematologic malignancies with promising results. Because some of the lentiviral vector components are cytotoxic, t
Stable Cell Lines for Production Gene Knock-out Cell Lines Service Why Develop Stable Cell Lines Stable cell lines can grow continuously over a long period of time without significant change in the expression level of the target gene. It is widely used in functional studies of genes, drug scree...
Construction of stable overexpression cell lines|过表达稳转细胞系构建 基本信息 更多信息 中文名称: 过表达稳转细胞系构建 中文同义词: 过表达稳转细胞系构建 英文名称: Construction of stable overexpression cell lines 英文同义词: Construction of stable overexpression cell lines CAS号: 分子式: 分子量: 0...
The cell line with stable silencing of the Mon1A has the advantages that the protein expression of the Mon1A is obviously reduced by suppressing the transcription of mRNA of the Mon1A; due to construction of the stable-expression cell line, not only can the expression state of the Mon1A of...
The invention relates to a related technology of medical molecular biology, and in particular relates to a cell research model constructed by adopting a siRNA jamming technology. A G6PD over-expressed ACHN stable cell strain disclosed by the invention is a G6PD over-expressed ACHN stable cell str...
Finally, monoclonal cell lines were prepared to obtain highly secreted foreign protein cell lines with stable properties. At present, the stable cell line of platform technology can reach >4g/L. Monoclonal The positive cell group obtained after transfection can be separated into single cells by ...
Here we describe a general method for construction of stable packaging cell lines that continuously produce lentiviral vectors. This uses Cre recombinase-mediated cassette exchange to insert a codon-optimised HIV-1 Gag-Pol expression ... KS Sanber,SB Knight,SL Stephen,... - 《Scientific Reports》...
followed by screening the polyclonal cell lines with hygromycin,subsequently screening the monoclonal cell line by limited dilution.The positive monoclonal cell lines were identified with reporter gene assay,thereafter the expression stability of luciferase was investigated in the positive cell lines.The ...
The proportion of amplicons that originated from each primer pair (marker) was stable across different 1st-primer concentrations (Fig. 2b and d). Obtaining sequences amplified by both the 1st and 2nd primers (here, amplicons distributed around 242–271 bp) is crucial for NGS. On the other ...