Our compressed Perturb-seq strategy relies on collecting measurements comprising random linear combinations of genetic perturbations, followed by deconvolving the perturbation effects on the transcriptome using sparsity-exploiting algorithms.doi:10.1038/s41587-023-02003-3Yao, Douglas...
The compressed Perturb-seq strategy measures small numbers of random composite samples, and decompresses these measurements to identify individual perturbation effects using the sparse structure of regulatory circuits, which substantially reduced the number of measurements and enhanced the screening efficiency....
Pooled CRISPR screens with single-cell RNA sequencing readout (Perturb-seq) have emerged as a key technique in functional genomics, but they are limited in scale by cost and combinatorial complexity. In this study, we modified the design of Perturb-seq by incorporating algorithms applied to rand...
Applying compressed Perturb-seq to genetic screensGene expression profilingHigh-throughput screeningdoi:10.1038/s41588-023-01613-6Wei LiNature Publishing Group USNature Genetics