Using an experimental and computational framework inspired by compressed sensing, we greatly reduced the number of measurements needed to run Perturb-seq. Our compressed Perturb-seq strategy relies on collecting measurements comprising random linear combinations of genetic perturbations, followed by deconvol...
Pooled CRISPR screens with single-cell RNA sequencing readout (Perturb-seq) have emerged as a key technique in functional genomics, but they are limited in scale by cost and combinatorial complexity. In this study, we modified the design of Perturb-seq by incorporating algorithms applied to rand...
Scalable genetic screening for regulatory circuits using compressed Perturb-seq Douglas Yao Loic Binan Brian Cleary Nature Biotechnology (2024) Highly sensitive spatial transcriptomics using FISHnCHIPs of multiple co-expressed genes Xinrui Zhou Wan Yi Seow Kok Hao Chen Nature Communications (2024) ...
Pooled CRISPR screens with single-cell RNA sequencing readout (Perturb-seq) have emerged as a key technique in functional genomics, but they are limited in scale by cost and combinatorial complexity. In this study, we modified the design of Perturb-seq by incorporating algorithms applied to ...
Applying compressed Perturb-seq to genetic screensdoi:10.1038/s41588-023-01613-6Wei LiNature GeneticsNature Publishing Group USNature Genetics