An intact SAM-dependent methyltransferase fold is encoded by the human endothelin-converting enzyme-2 gene A recent survey of protein expression patterns in patients with Alzheimer's disease (AD) has identified ece2 (chromosome: 3; Locations: 3q27.1) as the most... W Tempel,W Hong,L Dombrovs...
& Moss, B. Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions. J. Biol. Chem. 250, 9322–9329 (1975). CAS PubMed Google Scholar Malone, R. W., Felgner, P. L. & Verma, I. M. Cationic liposome-mediated RNA transfection. Proc. Natl...
We found 13i inhibits NSD3 activity with IC50 = 287 μM in vitro and suppresses the proliferation of JIMT1 breast cancer cells with GI50 = 36.5 μM, which express a high level of NSD3. Also, 13i downregulated the levels of H3K36me2/3 in a dose‐dependent manner. Our st...
Previous studies have shown that the catalytic SET domain of ASH1L is involved in the regulation ofHoxagenes in mouse embryonic stem (ES) cells5, suggesting that the histone methyltransferase activity of ASH1L is important for its gene activating function. Here, we demonstrate the relevance of th...
The RNA genomes of positive-strand RNA viruses, such as picornaviruses, alphaviruses and flaviviruses, have a dual function245. They act as an mRNA template for the instant translation of RNA-dependent RNA polymerase (RDRP) and as a genomic template for replication by the respective RDRP. The ...
context-dependent binding of certain COMPASS subunits to the complex, such as the NCOA6/ASC2 subunit that acts as an adapter for nuclear receptors (with or without ligands) and/or transcription factors [28]. The heterogeneity of cellular KCDCOMs (i.e., with or without NCOA6 or KDM6A) ...
H3K9 methyltransferase, also known as KMT1D; EP300: Enzyme p300; FSIQ: Full scale intelligence quotient; H3K4me1/2/3: Histone 3 lysine 4 mono/ di/tri methylationHATHistone acetyl transferase; H3R2me2: Histone 2 arginine 2 dimethylation; HDACi: Histone deacetylase inhibitors; Hox: Homeobox; ...
(2008) The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site. Nucleic Acids Res. 36, 1542-1554Taylor, A.B., Meyer, B., Leal, B.Z., Kotter, P., Schirf, V., Demeler, B., Hart, P.J., Entian, K.D., and ...
Unlike most radical SAM enzymes, the class?C radical SAM methyltransferase NosN binds two SAMs in the active site, using one SAM to produce a dAdo radical and the second as a methyl donor. Here, we report a mechanistic investigation of NosN in which an allyl analogue of SAM (allyl‐SAM)...
c o m /rev i ew s /d r u g d i s c REVIEWS Box 2 | Self-amplifying mRNA The RNA genomes of positive-strand RNA viruses, such as picornaviruses, alphaviruses and flaviviruses, have a dual function245. They act as an mRNA template for the instant translation of RNA-dependent RNA ...