PrmB methyltransferaseChloramphenicolTiamulinClindamycinThe genome of Azospirillum brasilense harbors a gene encoding S -adenosylmethionine-dependent methyltransferase, which is located downstream of an arsenate reductase gene. Both genes are cotranscribed and translationally coupled. When they were cloned and ...
Here we report the in vitro selection of a 33-nucleotide SAM-dependent methyltransferase ribozyme RNA from a randomized sequence. Detection and mapping of the methyl group on the RNA demonstrates site-specific methylation of the N7 position of guanine by SAM. The ribozyme is active over a wide...
SAM (dependent) I AM: the S-adenosylmethionine-dependent methyltransferase fold - ScienceDirect The S-adenosylmethionine-dependent methyltransferase enzymes share little sequence identity, but incorporate a highly conserved structural fold. Surprising... JL Martin,FM Mcmillan - 《Current Opinion in ...
Structural and mutational analysis reveals how nature evolves a classic methyltransferase active site into one that can serve as a dehydratase and a multifunctional pericyclase. Catalysis of both sets of reactions employs H133 and R295, two active-site residues that are not found in canonical ...
These data suggest that MAT2A activity is required for the m6A RNA methyltransferase-dependent control of protein synthesis. Moreover, supplementation of SAM led to the partial but significant rescue in the protein synthesis rate in response to MAT2A inhibition (Figures 6E and S6H). These data ...
Crystal structure of SAM-dependent methyltransferase from Pyrococcus horikoshii.doi:10.1107/S2053230X17016648METHYLTRANSFERASESPYROCOCCUS horikoshiiCRYSTAL structureMethyltransferases (MTs) are enzymes involved in methylation that are needed to perform cellular processes such as biosynthesis, metabolism, gene ...
Biochemistry Kinetics assay development of SAM-dependent methyltransferase and characterization of dimer formation of aged protein isoaspartyl methyltransferase (PIMT) PURDUE UNIVERSITY Alan M. Friedman ZhouZeyiAn adenosine deaminase-related growth factor homolog was isolated as a minor component in a ...
Crystal structure of SAM-dependent O- methyltransferase from pathogenic bacterium Leptospira interrogans. J Struct Biol 2007;159:523- 528. [PubMed: 17561415]Hou, X., Wang, Y., Zhou, Z., Bao, S., Lin, Y., and Gong, W. (2007) Crystal structure of SAM-dependent O-methyltransferase from...
Here we report the in vitro selection of a 33-nucleotide SAM-dependent methyltransferase ribozyme RNA from a randomized sequence. Detection and mapping of the methyl group on the RNA demonstrates site-specific methylation of the N7 position of guanine by SAM. The ribozyme is active over a wide...
However, some MTases show no methyltransferase activity. In the present study, the crystal structure of LepI, one MTase﹍ike enzyme isolated from A. flavus that catalyzes pericyclic reactions, was investigated to determine its structure‐function relationship. The overall structure of LepI in ...