The downfield 12 ppm assignment for Hγ reveals a Ser195-Oγ atom that is alkoxide-like in free enzyme. The exchange rates for His57-Hϵ2 and Ser195-Hγ at physiological pH values, 10sand 850 srespectively, are found to be catalytically competent. The results demonstrate capabilities...
— The time‐resolved tryptophyl fluorescence of α‐chymotrypsinogen A and α‐chymotrypsin in the crystalline state and in buffer solution at room temperature was analyzed globally. Tripleexponential decay functions are necessary to adequately describe the tryptophyl fluorescence decay surfaces of the ...
This behavior, in a protein where the exposure of the tryptophans to solvent is minimal, is in marked contrast to that observed in bovine serum albumin where at least one of the two tryptophans can interact directly with water. Get PDF (219K) More content like this Find more content: ...
The downfield 12 ppm assignment for Hgamma reveals a Ser195-Ogamma atom that is alkoxide-like in free enzyme. The exchange rates for His57-Hepsilon2 and Ser195-Hgamma at physiological pH values, 104 s-1 and 850 s-1 respectively, are found to be catalytically competent. The results ...