5. 3-Chymotrypsine-like protease The extensive process of proteolysis releases the functional polypeptides which are mainly achieved by the main proteinase and are also frequently named 3C-like proteinase (3CLpro). This indicates a similar cleavage site with the early picornavirus of 3C proteinases ...
Herein, we have identified potential drugs that target the 3-chymotrypsin like protease (3CLpro), the main protease that is pivotal for the replication of SARS-CoV-2. Computational molecular modeling was used to screen 3987 FDA approved drugs, and 47 drugs were selected to study their ...
In addition to the papain-like protease, the 3-chymotrypsin-like protease (3CLpro), also known as the main protease, is important for the posttranslational processing of SARS-CoV-2 polypeptides and the production of 16 non-structural proteins (nsps)5,11,12. The nsps play fundamental roles ...
The present study investigated the binding affinity of phytocompounds with 3-Chymotrypsin-like (3CLpro) and Papain-like proteases (PLpro) of SARS-CoV2 using in-silico techniques. A total of 32 anti-protease phytocompounds were investigated for the binding affinity to the proteins. Docking was ...
3-Chymotrypsin-like protease (3CLpro) is one such protease. The cleavage of SARS-CoV-2 polyproteins by 3CLpro is facilitated by a Cys145-His41 catalytic dyad. We here characterized the catalytic roles of the cysteine-histidine pair for improved understanding of the 3CLpro reaction mechanism...
The 3-chymotrypsin-like protease (3CLpro) is a validated molecular target for COVID-19, and it is key for developing potent and selective inhibitors for inhibiting viral replication of SARS-CoV-2. In this review, we discuss structural relationships and diverse subsites of 3CLpro, shedding ...
Describes the identification, characterization and purification of two serine proteases like chymotrypsin, called chymase and multicatalytic protease . Chymase and the multicatalytic protease are both proof of an enzymatic activity capable of cleaving between the methionine and L-aspartic acid, an activity...
本研究组前期采用抑制性差减杂交,构建差减文库及斑点杂交,发现一个Chymotrypsin(CHY)基因在致倦库蚊Culex pipiens quinquefasciatus对硫磷抗性品系中高表达.用BliastX软件在NCBI上进行比对,发现高表达的CHY与XM_001843342具有98.58%同源性.在此基础上,利用PCR技术扩增出CHY884bp片段,开放阅读框为849bp,预测编码282个...
In vitro assay proved that TET inhibits the chymotrypsin-like (CT) activity of purified human 20S proteasome in a dose-dependent manner with IC 50 of 0.8μmol/L. After exposed to TET for 24h, the CT activity in human prostate cancer PC-3 cells was inhibited with IC 50 of 35μmol/L,...
3-Chymotrypsin-like protease (3CLpro), the main protease in SARS-CoV-2, plays an indispensable role in the SARS-CoV-2 viral life cycle by cleaving the viral polyprotein to produce 11 individual non-structural proteins necessary for viral replication. 3CLpro is one of two proteases that ...