Other human pancreatic proteases such as chymotrypsin B1, chymotrypsin B2, chymotrypsin-like enzyme-1, elastase 2A, elastase 3A, or elastase 3B are inactive or markedly less effective at promoting procarboxypeptidase activation. On the basis of these observations, we propose that CTRC is a ...
Lesk AM, Fordham WD: Conservation and variability in the structures of serine proteinases of the chymotrypsin family. J Mol Biol. 1996, 258: 501-537. Article CAS PubMed Google Scholar Johnson SC, Ewart KV, Osborne JA, Delage D, Ross NW, Murray HM: Molecular cloning of trypsin cDNAs and...
Recently, we found that limited proteolysis with chymotrypsin converts rPfSUB1p54 to a soluble, enzymatically active core catalytic domain of 38 kDa (called rPfSUB1cat) still partially bound to a 9-kDa prodomain fragment (Prodp9) but with no modification of its substrate specificity10. This ...
Purification of trypsinogens yielded a single peak with an N-terminal amino-acid sequence of LPIDD. Cloning of pretrypsinogen cDNAs revealed 2 distinct but nearly identical isoforms. At the amino acid level, the only difference between the 2 isoforms is an Ala/Ser change at position 15 within...
Our results demonstrate that Hap belongs to the SA (chymotrypsin) clan and contains a catalytic triad conserved among a subfamily of autotransporter serine proteases. Identification of the three alternate autoproteolytic cleavage sites in Hap revealed a consensus target sequence for Hap enzymatic activity...
Explain why a reactant is also called the "substrate" when it comes to enzymatic reactions. Protease due its function demonstrates a self-digesting potential. Why self-digesting is less severe for chymotrypsin than it is for trypsin? Explain. ...
it has not been known whether it has this role throughout the gastrointestinal tract (GIT). Here, we show that AMCase is a major chitinase in stomach resistant to pepsin and trypsin/chymotrypsin digestion and that it can function as a digestive enzyme under conditions also in other parts of...
The degradation products empty from the stomach into the small intestine. Since the products of pepsin are only partially cleaved, they are polypeptides. These molecules are too large to be absorbed by the intestinal cells. They are further degraded by chymotrypsin, trypsin, and specific enzymes th...
A) Amylase B) Lipase C) Trypsin D) Pepsin E) Chymotrypsin. The enzyme that is the most potent digester of fats is 1) aminopeptidase 2) pancreatic lipase 3) lingual lipase 4) maltase 5) nucleosidase The large intestine secretes ___ but no digestive enzymes. The normal pH range of ...
A proteomics approach was therefore employed to identify exposed Cys residues in Sb9. Recombinant human Sb9 was treated with the alkylating agent iodoacetimide, then digested by trypsin or chymotrypsin, and analyzed by mass spectrometry. The resulting tryptic and chymotryptic peptides collectively ...