Chromatin-associated molecular patterns (CAMPs) Chromatin is a structural complex of DNA with various basic and acidic proteins. Histones are basic, positively charged proteins that associate with negatively charged DNA, thereby organizing DNA into structures called nucleosomes [27,28]. A nucleosome con...
Driven by recent technological innovations for detecting chromatin-associated RNAs, diverse roles are being revealed for these RNAs and associated RNA-binding proteins (RBPs) in gene regulation and genome function. Such functions include locus-specific roles in gene activation and silencing, as well ...
但是,在人类基因组中编码的1,600多种转录因子中,只有一小部分被检测了。 这篇文章作为ENCODE项目的一部分,使用人类使用人类Hepg2细胞系中的208个染色质相关蛋白质(CAP)进行高通量测序,然后对得到的染色质免疫沉淀数据进行处理和分析。本文旨在尽可能完整的概述这种细胞类型的基因调控网络。 二、数据来源 ...
Furthermore, using a radioimmunoassay system, we have estimated that fibronectin represents about 0.7% of the total protein in both chromatin preparations and whole fibroblasts. Using a nitrocellulose filter assay system, we also show that fibronectin is a DNA-binding protein having an equilibrium ...
This class of RNA was initially designated fraction B (chromatin-associated RNA). In the characterization of fraction B, human myeloma cells labeled for 3 and 24 h were fractionated into subcytoplasmic and subnuclear components and the [ 3H]-RNA was differentially extracted. After 3 and 24 h ...
Roles for protein kinases in the nucleus, indeed directly on chromatin, are emerging as important in mediating gene expression. Sutcliffe et al. show that protein kinase C-θ (PKC-θ) joins this group of chromatin-associated kinases and functions in inducible gene expression in T cells. PKC-θ...
High-mobility-group (HMG) proteins are small and relatively abundant chromatin-associated proteins, which act as architectural factors. In plants, two groups of chromosomal HMG proteins have been identified, namely the HMGA family, typically containing four A/T-hook DNA-binding motifs, and the HMGB...
To gain some insight into the mechanism of regulation of gene expression by estradiol in chick oviduct, the effect of steroid administration in vivo on the size distribution of nascent RNA transcripts synthesized in isolated 'chromatin' in vitro was analyzed. In 'chromatin' from withdrawn (control...
1. 1. Three endonucleases designated A 1, A 2 and B have been detected in and purified from chromatin of germinating barley. 2. 2. The enzymes are similar in a number of catalytic properties such as optimal activity at near-neutral pH, capacity for hydrolysis of both RNA and DNA, inabil...
Chromatin-associated glycoproteins of normal rat liver and Novikoff hepatoma ascites cells. 来自 NCBI 喜欢 0 阅读量: 26 作者:AH Goldberg,LC Yeoman,H Busch 摘要: Glycoproteins were demonstrated in the 0.6 M NaCl extract of chromatin of normal liver and Novikoff hepatoma cells by periodic acid-...