Current methods to identify sites of RNA interaction with the genome are limited to the study of a single RNA at a time. Here we describe a protocol for ChAR-seq, a strategy to identify all chromatin-associated RNAs and map their DNA contacts genome-wide. In ChAR-seq, proximity ligation ...
学术范收录的Journal Chromatin-associated RNAs as facilitators of functional genomic interactions,目前已有全文资源,进入学术范阅读全文,查看参考文献与引证文献,参与文献内容讨论。学术范是一个在线学术交流社区,收录论文、作者、研究机构等信息,是一个与小木虫
Author Correction: Chromatin-associated RNAs as facilitators of functional genomic interactions 来自 国家科技图书文献中心 喜欢 0 阅读量: 146 作者:X Li,XD Fu 摘要: Mammalian genomes are extensively transcribed, which produces a large number of both coding and non-coding transcripts. Various RNAs are...
Finally, AGO2 RIP-seq experiments reveal that, AGO2 is strongly enriched in small-RNAs encompassing promoter as well as other parts of heat shock and other gene loci on both sense and antisense, with a strong bias for antisense, particularly after heat shock. Taken together our results reveal...
Figure 6. SAF-A Oligomerization Regulates Interphase Chromosome Structure via Chromatin-Associated RNAs (A) Top: Model for chromatin decompaction by SAF-A oligomerization. Bottom: Experimental strategy to test model. Flp-In T-REx 293 cells depleted of endogenous SAF-A (48 hr) and then doxycycline...
Reduction of H3K27me3 levels or elimination of RNAs had no effect on the association of the components of PcG and TrxG complexes to DNA. Using a combination of proximity ligation assay-based techniques and super-resolution microscopy, we found that the number of protein–DNA and RNA–DNA ...
(cf) RNAs, microRNAs, telomeric repeat-containing RNA (TERRA), extracellular traps (ETs), and RNA- or DNA-binding proteins can also be included in this group, given their similar origins’ contribution to inflammation and organ dysfunction in sepsis. Increased levels of CAMPs, i.e., cfRNA,...
PARP1-bound RNAs were eluted from membrane in c, deproteinized, ligated to 3′ and 5′ adapters (lane 4). Lanes 1 and 6 are control 19-mer and 24-mer labeled RNAs, ligated to 3′ adapter. These ligated 3′ adapter control RNAs were further ligated to 5′ adapters (lanes 2 and 7...
small RNAsgene expressionepigeneticsRNA-dependent RNA polymerase2 (RDR2)Though the mechanisms governing nuclear organizatiThelma F MadzimaJi HuangKaren M McGinnisEpigenetics: official journal of the DNA Methylation SocietyMadzima TF, Huang J, McGinnis KM (2014) Chromatin structure and gene expression ...
A plant-specific DNA-dependent RNA polymerase IV (Pol IV) generates small (∼30–40 nt) transcripts predominantly from TEs and repeats to initiate RdDM. These RNAs get converted into double-stranded (ds) forms by RNA DEPENDENT RNA POLYMERASE2 (RDR2). The ds substrates are processed into...