Expanding the repertoire of plasmids for PCR-mediated epitope tagging in yeast Yeast, 25 (2008), pp. 287-292 10.1002/yea.1581 18338317 CrossrefView in ScopusGoogle Scholar 46 Cullen P.J. Biofilm/Mat assays for budding yeast Cold Spring Harb. Protoc, 2015 (2015), pp. 172-175 10.1101/pdb...
Using ChIP-seq with an antibody targeting an N-terminal epitope of CHD8 (Supplementary Fig. 2a,b), we identified CHD8-binding sites in H9-derived human NSCs and human midfetal brain at 16–19 post conception weeks (PCWs). As described above, potential ASD risk genes converge in co-...
AP-MS is often performed by expressing the protein of interest, termed the bait, fused to an epitope tag that facilitates affinity purification, although this approach is also amenable to the analysis of endogenous baits if appropriate antibodies are available [2]. Following cell lysis, antibodies...
A plant-specific DNA-dependent RNA polymerase IV (Pol IV) generates small (∼30–40 nt) transcripts predominantly from TEs and repeats to initiate RdDM. These RNAs get converted into double-stranded (ds) forms by RNA DEPENDENT RNA POLYMERASE2 (RDR2). The ds substrates are processed into ...
A c-terminal v5 epitope tag was added to aid in detection. The cDNA was then cloned into the pPyCAG iH vector ([68], hygromycin resistance) for expression under a ubiquitous (CAG) promoter. CJ9 ESCs were electroporated with the linearized plasmid in the presence of hygromycin and ...
However, it has been previously reported that early Drosophila embryos lack histone H1 =-=[28]-=-, opening the possibility that anti-H1 antibodies could cross-react with a non-H1 epitope. To directly analyse paternal chromatin assembly in sra eggs, we used antiacetylated-H4 antibodies. In ...
A high-performance thioredoxin-based scaffold for peptide immunogen construc- tion: proof-of-concept testing with a human papillomavirus epitope. Sci Rep 2014; 4:4729. 32 Wu C, Liu T, Chen W, et al. Redox regulatory mechanism of transnitrosylation by thioredoxin. Mol Cell Proteomics 2010; 9...
A critical epitope for substrate recognition by the nucleosome remodeling ATPase ISWI Nucleic Acids Res., 30 (2002), pp. 649-655 View in ScopusGoogle Scholar Corona et al. 2002 D.F. Corona, C.R. Clapier, P.B. Becker, J.W. Tamkun Modulation of ISWI function by site-specific histone ...
In an effort to gain insight into the biochemical functions of FVE, we overexpressed FVE tagged with the hemagglutinin (HA) and FLAG epitope at the N-terminus in acg1 mutants. The results of physiological and molecular analyses demonstrated that FVE overexpression in acg1 rescued the mutant ...