BET inhibitors, such as JQ1, function by competitively binding to acetylated histone lysine residues to prevent interactions between BET proteins and chromatin. Although these drugs have gained notable attention as anti-tumor agents in the treatment of certain cancers37,38,39,40, as well as with ...
(D) Enrichment for genetic associations (p value < 10−6) in physically interacting regions as a function of the minimal distance between the SNP-distal peak pair. Enrichments and 95% confidence intervals are calculated using Fisher’s exact test. Since regions that are proximal in linear gen...
correlation coefficient.k, RNA reads from RNA-seq, iMARGI and MUSIC mapped to both strands (+ and −) in human H1 cells. Ensemble and individual MUSIC data from three single cells are shown.l, Distribution of chromatin-associated pre-mRNA and nsaRNA on chromosome 1 as measured by ensem...
但是,在人类基因组中编码的1,600多种转录因子中,只有一小部分被检测了。 这篇文章作为ENCODE项目的一部分,使用人类使用人类Hepg2细胞系中的208个染色质相关蛋白质(CAP)进行高通量测序,然后对得到的染色质免疫沉淀数据进行处理和分析。本文旨在尽可能完整的概述这种细胞类型的基因调控网络。 二、数据来源 ...
Three-dimensional (3D) chromatin structure is closely related to genome function, in particular transcription. However, the folding path of the chromatin fiber in the interphase nucleus is unknown. Here, we systematically measured the 3D physical distance between pairwise labeled genomic positions in ...
To test the function of the conserved CTCF motif in host-integrated HPV16, we performed ATAC-seq, CTCF ChIP-seq, and RNA-seq on 5 HPV16+ cell lines: 93-VU147T [28] (7 integration sites), CaSki [29] (6 integration sites), HMS-001 [30] (1 integration site), SCC-090 [31] (1 ...
A comparative atlas of single-cell chromatin accessibility in the human brain 发表期刊和时间:Science,13 October 2023 Lab:Bing Ren, 实验室正在研究正常和癌症细胞的基因调节机制,与路德维希研究所和加州大学合作密切。 摘要:探索了三名成年人42个大脑区域110万个细胞的开放染色质景观。整合这些数据揭示了107种...
(ETH Zurich) sought to understand a significant challenge to maintaining weight loss – the retention of an “obesogenic” memory – via single-cell transcriptomic/epigenetic analyses in human/mouse adipose tissues before and during obesity and after weight loss. Their findings – that adipose tissue...
Similarly, in human cells the function of the hCDC47 form does not appear to be compensated for by other members (29). On the other hand, yeast MCMs exhibit genetic interactions (4, 7) and, just recently, have been shown to physically associate with one another (30). In Xenopus or...
Then, we used the ChromHMM OverlapEnrichment function to calculate the overlap fold enrichment between each human state (liftedOver to mm10) and each mouse state. For each pair of human-mouse states, we determined whether the pair of states correspond to a one-to-one mapping by checking ...