为了确保实验结果的可重复性,至少重复两次ChIP-seq实验并检查reads和鉴别的peaks的重复性。 读长的可重复性可通过计算每个基因组位置的(mapped) read conuts的Pearson correlation coefficient来得出
complicated data manipulation.Lasergene Genomicsmakes it quick and easy to set up your ChIP-Seq analysis project, by offering an easy-to-use wizard that guides you through project setup in minutes. The software also includes several different customizable options for variant detection...
We have designed software called Time-Dependent ChIP-Sequencing Analyser (TDCA), which is the first program to automate analysis of time-dependent ChIP-seq data by fitting to sigmoidal curves. We provide users with guidance for experimental design of TDCA for modeling of time course (TC) ChIP-...
之前看到有一些大佬在分析数据时使用了CEAS软件,看起来很好用:CEAS(ChIP-Enrichment Analysis Suite)是一种用于分析和解释 ChIP-seq 数据的工具套件,旨在识别和量化蛋白质-DNA 结合的富集区域及其生物学意义。CEAS 专注于 ChIP-seq 数据的后续分析和解读,包括在基因组中的富集模式、功能注释以及与其他数据集的比较。
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In addition to implementing previously published ChIP–microarray (ChIP-chip) analysis methods, the software contains statistical methods designed specifically for ChlP sequencing (ChIP-seq) data obtained by coupling ChIP with massively parallel sequencing. The modular design of CisGenome enables it to ...
这篇2011年的文章对ChIP-Seq的分析流程进行比较详细的讲解,个人读完后对ChIP数据质量控制和可信度提高上有了更多的认识,但之后的call-peak和peak注释作者主要是介绍一款名为CisGenome的软件计算原理和优点,然而…
ChIP-seq基本流程及工具 ChIP-seq数据分析整理 1.Alignment 2.Peak detection 3.Peak annotation 1. GO analysis 2. Pathway analysis 4.Differential analysis 5.Peak gene 6.Pathway analysis 7.Data visualization 1.Alignment base calling——Off-Line Basecaller software (OLB V1.8.0)....
ChIP-seq基本流程及工具 ChIP-seq数据分析整理 1.Alignment 2.Peak detection 3.Peak annotation 1. GO analysis 2. Pathway analysis 4.Differential analysis 5.Peak gene 6.Pathway analysis 7.Data visualization 1.Alignment base calling——Off-Line Basecaller software (OLB V1.8.0)....
Unlock the power of ChIP-seq data analysis with our comprehensive course, Learn ChIP-seq Data Analysis Using Bioinfo Linux Pipeline. Whether you’re a beginner or looking to expand your bioinformatics skills, this course provides a step-by-step guide to mastering ChIP-seq analysis using essential...