Kent SB. Chemical synthesis of peptides and proteins. Annu Rev Biochem. 1988; 57:957-989. Epub 1988/01/01. doi: 10.1146/annurev.bi.57.070188.004521 PMID: 3052294Kent (1988) " Chemical Sy8nthesis of Peptides and Proteins, " Annu. Rev. Biochem. 57:957-989....
For the proteins that cannot be expressed exactly by cell expression technology (e.g., proteins with multiple posttranslational modifications or toxic proteins), chemical synthesis is an important substitute. Given the limited peptide length offered by solid-phase peptide synthesis invented by Professor...
D. and SANTERRE, R. F. (1991), ChemInform Abstract: Synthesis of Peptides and Proteins by Chemical and Biotechnology Means. ChemInform, 22: no. doi: 10.1002/chin.199125275 Author Information Eli Lilly and Co., Indianapolis, IN, USA Publication History Issue published online: 23 AUG 2010 ...
Effective automated SPPS is key to the synthesis of peptide building blocks for chemical protein synthesis, and for the production of peptides and small proteins for pre-clinical studies. In the research laboratory, the total synthesis of proteins based on modern chemical ligation methods has ...
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This protocol provides a detailed procedure for the chemical synthesis of proteins through native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) the solid-phase synthesis of peptide hydrazides via Fmoc chemistry and (ii) the native chemical ligation of pepti...
proteins expressed in eukaryotic cells. We insert synthetic peptides into GFP, NaV1.5 and P2X2 receptors via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating protein function. We anticipate the approach will overcome some drawbacks of ...
synthesismetabolism代谢chemical合成bioregulators III.ChemicalCharacteristicsand SynthesisofBioregulators •Aminoacids,amines,peptidesandproteins •SteroidBioregulators •Thyroidhormones •Eicosanoids •Otherimportantregulators ChemicalNatureofHormones •Peptideandproteinhormones(mostabundant):thyrotropin releasinghor...
Pushing the Boundaries of Chemical Protein Synthesis: The Case of Ubiquitin Chains and Polyubiquitinated Peptides and ProteinsPushing the Boundaries of Chemical Protein Synthesis: The Case of Ubiquitin Chains and Polyubiquitinated Peptides and Proteinsdesulfurizationpeptide...
Chemical protein synthesis can enable the preparation of proteins containing post-translational modifications or unnatural variations such as D-amino acids. Such modified proteins are not easily fabricated by other methods. This Review provides an overvi