The maipulation of protein structure enables a better understanding of the principles of protein folding, as well as the developement of novel therapeutics and drug-delivery vehicles. Chemical synthesis is the most powerful approach for constructing proteins of novel design and structure, allowing for ...
protein chemical synthesisthioester synthesismultiple fragment condensationprotein probesprotein biopharmaceuticsIntroductionBrief HistoryCurrent TechnologyApplicationsConclusion and PerspectivesReferencesComment 1Comment 2Referencesdoi:10.1002/9783527664801.ch6Lei Liu...
This protocol provides a detailed procedure for the chemical synthesis of proteins through native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) the solid-phase synthesis of peptide hydrazides via Fmoc chemistry
Sakakibara, S (1999) Chemical synthesis of proteins in solution. Biopolymers 51: pp. 279-296Sakakibara S (1999) Chemical synthesis of proteins in so- lution. Biopolymers 51:279-296.Sakakibara, S. (1999) Chemical synthesis of proteins in solution. Biopolymers (Peptide Sci.) 51, 279-296...
This protocol provides a detailed procedure for the chemical synthesis of proteins through native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) the solid-phase synthesis of peptide hydrazides via Fmoc chemistry and (ii) the native chemical ligation of pepti...
For the proteins that cannot be expressed exactly by cell expression technology (e.g., proteins with multiple posttranslational modifications or toxic prot
Chemical synthesis of proteins using hydrazide intermediates Protein chemical synthesis offers useful and otherwise-difficulty-to-obtain biomacromolecules for biological and pharmaceutical studies. Recently, the hydr... Huang, Yi-Chao,Fang, Ge-Min,L Liu - 《National Science Review》 被引量: 6发表: 20...
The product of complete length the protein which is produced by the synthesis of cell free chemically is identical. If the concatenated product of complete length is allowed, rihuorudo and/or it is possible to be oxidized, the protein numerator of natural die disulfide content forms. Although ...
3. Synthesis前兆群体异常信息提取与地震短期预测研究 4. A combined IR and XRD study of natural well crystalline goethites(α-FeOOH) 5. 基于鲁棒μ-synthesis的多输入多输出振动主动控制实验 6. Chemical characteristics of rainwater in karst rural areas, Guizhou Province, Southwest China 7. Synthe...
Synthesis of proteins by native chemical ligation A simple technique has been devised that allows the direct synthesis of native backbone proteins of moderate size. Chemoselective reaction of two unprotect... P Dawson,T Muir,I Clark-Lewis,... - 《Science》 被引量: 4647发表: 1994年 ...