A small library of conformationally-restricted, 2-, 3- and 4-fold multivalent nitrilotriacetic (NTA) ligands have been developed to bind proteins bearing polyhistidine tags in a symmetry-guided manner in the presence of Ni(II). These reagents seek to control protein:ligand speciation and promote...
In May 2024, ‘Protein Degradation in Focus’, a special symposium aimed at bringing together scientists in the targeted protein degradation (TPD) field, was held in Dundee, UK. David Zollman and Kirsten McAulay report on the topics discussed and the exciting outlook for science in the TPD ...
We also disclosed that Au8 clusters can protect Lys VI activity against denaturation and act as a fluorescent probe to detect thimerosal in vaccines.II. Fluorescein-5-isothiocyanate-conjugated protein-directed synthesis of gold nanoclusters for fluorescent ratiometric sensing of an enzyme–substrate ...
First, the structural and functional features of p27 are well understood20–23, providing an ideal model system for studying small molecule:disordered protein interactions. Second, the ability to chemically modulate p27 function would be beneficial in several bio- logical settings. For example, p27...
The specificity and ease with which they function in each of their bio-functions appear to be largely governed by the nature and disposition of the protein globule around the porphyrin reaction center. Synthetic porphyrin frameworks confined within or around a pre-organized molecular entity like the...
granzyme B-mediated killing was detected earlier in the assay for γδD4compared to γδD14T cells (Fig.3f). The time interval between the fluorescent protein diffusion into the nucleus and the actual death of the target cell (phase iv-v in Fig.3b) was shorter for γδD4compared to ...
NTP and adenosine triphosphate (ATP) have a similar structure and competes with each other to bind to the viral RdRp with nearly same efficiencies, therefore NTP is used by the viral RdRp which results in premature termination of RNA synthesis, halting the growth of the RNA strand after a ...
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, and probably the most important cattle disease in terms of economic i... Y Xie,QS Guo,GS Wang - Molecules 被引量: 0发表: 2016年 Barley beta-glucan-protein based bioplastic film with enhanced physicochemica...
7and the scaffold has been engineered for improved synthesis and solubility.8Affibody molecules can be selected to a large variety of different proteins, and can be functionalized with genetic fusions to protein modules9,10or by chemical conjugation to toxic molecules,11reviewed in Löfblomet al....
Functionalisation of a POI ligand at a position that does not hinder binding to the target protein is of essential importance for the generation of successful PROTACs. Pleasingly, analysis of published X-ray structures of successful LSF substrates in complex with their protein targets revealed that...