Principles of peptide synthesis: (Reactivity and Structure Series, Vol. 16). By M. Bodansky, Springer-Verlag, Berlin/New York, 1983. 240 pp. $49.00doi:10.1016/0003-2697(84)90581-5WilliamS.HancockElsevier Inc.Analytical Biochemistry
We will use the Kaiser test, a qualitative method to test for the presence of primary amines, to monitor the progress of the reaction. Figure 1. Concept behind the solid phase peptide synthesis (SPPS). Subscription Required. Please recommend JoVE to your librarian. Procedure 1. Loading the ...
Cancer is the second leading cause of death affecting nearly one in two people, and the appearance of new cases is projected to rise by >70% by 2030. To effectively combat the menace of cancer, a variety of strategies have been exploited. Among them, the development of peptide–drug conjug...
The importance of peptide self-assembling building blocks has been recognized in the last years, as demonstrated by the literature available on the topic. The simple structure of peptides, as well as their facile synthesis, makes peptides an excellent family of structural units for the bottom-up...
Satellite DNA are simple non-coding tandem repeats that are widespread and abundant in eukaryotic organisms[1]. They are primarily present as vast tracts (105– 107bp) at the centromeric and pericentromeric (flanking the centromere) heterochromatin of eukaryotic chromosomes. Although centromeres are sp...
Peptide level turnover measurements enable the study of proteoform dynamics. Mol. Cell. Proteomics 17, 974–992 (2018). CAS PubMed PubMed Central Google Scholar Kristensen, A. R., Gsponer, J. & Foster, L. J. Protein synthesis rate is the predominant regulator of protein expression during...
A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast. EMBO J. 23, 3825–3835 (2004). CAS PubMed PubMed Central Google Scholar Brasher, S. V. et al. The structure of mouse HP1 suggests a unique mode of single peptide recognition by the ...
Rheumatoid arthritis (RA) is an autoimmune disease with multifactorial etiology and intricate pathogenesis. In RA, repeated monotherapy is frequently associated with inadequate efficacy, drug resistance, and severe side effects. Therefore, a shift has oc
are then cleaved to prohormones in the ER. The prohormone is then cleaved to the active hormone in the Golgi. These hormones may also serve as neurotransmitters. Calcitonin, a peptide hormone secreted by parafollicular cells of the thyroid gland, participates in the regulation of blood Ca2+...
Targeted proteomics methods like parallel reaction monitoring, in contrast, typically measure the levels of a handful of specific peptides (7) and are therefore conceptually more akin to existing clinical tests. Consequently, targeted proteomics does not raise the same ethical issues as system-wide ...