Cell cycleTo investigate the correlation between subnucleolar structure and function, the precise distribution and configuration of nucleolar DNA during the cell cycle of Allium sativum were determined using the NAMA-Ur DNA-specific staining technique. We showed that nucleolar DNA is present in two ...
Despite variation in methylation status through the life cycle, rRNA genes contained in the B-NOR were never observed to be active, which indicates that DNA methylation is not the direct cause of their inactivity. Other causes such as chromatin structure or repression by genes on A chromosomes ...
and J. Hillova: DNA synthesis and changes in radiosensitivity during the cell cycle of vegetative culture of Chlamydomonas reinhardi . Int. J. Radiat. Biol. 17 (2), 127–134 (1970).Cechacek, Z. and J. Hillova. DNA synthesis and changes in radiosensitivity during the cell cycle of ...
The results indicate that ADP-ribosylation may be involved in DNA replication and cell cycle progression. It was also found that the DNA-damaging agents, MNNG, MMS and 4NQO could lower cellular NAD content in a dose-dependent way. This DNA-damage-induced NAD lowering could be partially or ...
CHO cells were grown in the presence of 1 μ M CdCl 2 and subjected to ATP-dependent replicative DNA synthesis after permeabilization. By decreasing the density of the cell culture replicative DNA synthesis was diminishing. At higher than 2 × 10 6 cell/ml concentration Cd had virtually no ef...
Mass spectrometry-based methods can provide a global expression profile and structural readout of proteins in complex systems. Preserving the in vivo conformation of proteins in their innate state is challenging during proteomic experiments. Here, we int
Of note, NAD+ levels are strongly affected by massive DNA damage and subsequent PARP1 hyperactivation, as we have shown in freshly isolated PBMC [45], but NAD+ levels and PARP1 activity both vary during the cell cycle with NAD+ levels being lowest during S/G2 phase, possibly due to ...
Offspring of Mothers Fed a High Fat Diet Display Hepatic Cell Cycle Inhibition and Associated Changes in Gene Expression and DNA Methylation. Offspring of Mothers Fed a High Fat Diet Display Hepatic Cell Cycle Inhibition and Associated Changes in Gene Expression and DNA Methylation... - 《Plos One...
(1973) Periodic changes in the association of mammalian DNA with the membrane during the cell cycle. Nature new Biology 243: pp. 227-230Yamada, M., Hanaoka, F. 1973. Periodic changes in the association of mammalian DNA with the membrane during the cell cycle. Nature, New Biol. 243 :227...
CellTagging relies on single-cell capture of CellTags—heritable DNA barcodes expressed as polyadenylated transcripts7,16,17. In the standard workflow, CellTags are captured as transcripts and reverse transcribed (RT), along with cellular mRNA, during 3′ end scRNA-seq library preparation. In co...