近日，国家癌症中心/中国医学科学院肿瘤医院焦宇辰课题组、赵宏课题组和曲春枫课题组合作在Science Translational Medicine发表了题为 Simultaneous analysis of mutations and methylations in circulating cell-free DNA for hepatocellular carcinoma detection 的研究论文。研究团队开发了一种名为MCP（Mutation Capsule Plus）...
近日,国家癌症中心/中国医学科学院肿瘤医院焦宇辰课题组、赵宏课题组和曲春枫课题组合作在Science Translational Medicine发表了题为Simultaneous analysis of mutations and methylations in circulating cell-free DNA for hepatocellular carcinoma ...
Enzymatic methylation sequencing can be used for more effective detection of methylation patterns in cell-free DNA (cfDNA).
">原文解读:APOBEC-coupled epigenetic sequencing(ACE-Seq)( 点击阅读) 案例4:cfhMeDIP-seq对5-羟甲基胞嘧啶(5hmC)特征进行检测以预测早期胰腺癌 此研究利用cfDNA hMeDIP-seq对胰腺导管腺癌(PDAC)队列(n=64)与非癌症队列(n=243)的5hmC变化进行比较分析。在数千个基因中鉴定差异性羟甲基化,结果表明胰腺发育...
首先是全基因组亚硫酸氢盐测序(Whole-genome bisulfite sequencing,WGBS),它可以对 cfDNA 的甲基化特征进行分析。其次是利用特定的算法来识别差异甲基化位点(Differentially methylated loci,DMLs)和差异甲基化区域(Differentially methylated regions,DMRs) 。最后,通过血浆 DNA 甲基化反卷积分析来确定不同组织和细胞类型对...
[4].Sun K, Jiang P, Chan KC, et al. Plasma DNA tissue mapping by genome-wide methylation sequencing for noninvasive prenatal, cancer, and transplantation assessments. Proc Natl Acad Sci U S A. 2015;112(40):E5503-E5512. doi:10.1073/pnas.1508736112 ...
11. Liang W, Zhao Y, Huang W, et al. Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA). Theranostics 2019; 9:2056-70. 12. ...
Clinical validation of a multicancer detection blood test by circulating cell-free DNA (cfDNA) methylation sequencing: The THUNDER study.doi:10.1200/JCO.2022.40.16_suppl.10544544333210544#Background:The development of ultra-sensitive genomic and epigenomic assays enables the early detection of ...
Molecular Biologist: Specialize in the molecular mechanisms of DNA methylation, including the enzymes and pathways involved in cfDNA methylation and their impact on gene expression. Clinical Oncologist: Concentrate on the clinical applications of cfDNA methylation, including its use as a biomarker for can...
③ Gao Y, et al. Whole-genome bisulfite sequencing analysis of circulating tumour DNA for the detection and molecular classification of cancer. Clin Transl Med. 2022 Aug;12(8):e1014. doi: 10.1002/ctm2.1014. PubMed PMID: 35998020. ④ Hua X, et al. Tumor detection by analysis of both sym...