this system replaces bisulfite sequencing with a series of enzymatic conversions that ultimately convert unmethylated cytosine bases into uracil6. In relying on enzymes rather than bisulfite salts, the EM-seq protocol minimizes damage to the DNA and enables up to 15% more methylation detection ...
近日,国家癌症中心/中国医学科学院肿瘤医院焦宇辰课题组、赵宏课题组和曲春枫课题组合作在Science Translational Medicine发表了题为Simultaneous analysis of mutations and methylations in circulating cell-free DNA for hepatocellular carcinoma detection的研究论文。研究团队开发了一种名为MCP(Mutation Capsule Plus)的方法...
Vaisvila, Romualdas, et al. “Enzymatic Methyl Sequencing Detects DNA Methylation at Single-Base Resolution from Picograms of DNA.” Genome Research, 17 June 2021, genome.cshlp.org/content/early/2021/06/17/gr.266551.120, 10.1101/gr.266551.120. Accessed 30 Sept. 2021....
近日,国家癌症中心/中国医学科学院肿瘤医院焦宇辰课题组、赵宏课题组和曲春枫课题组合作在Science Translational Medicine发表了题为Simultaneous analysis of mutations and methylations in circulating cell-free DNA for hepatocellular carcinoma ...
[2]Gao Q, Lin YP, Li BS, et al. Unintrusive multi-cancer detection by circulating cell-free DNA methylation sequencing (THUNDER): development and independent validation studies. Ann Oncol. 2023;34(5):486-495. doi:10.1016/j.annonc.2023.02.010 ...
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[3]Gao Q, Lin Y P, Li B S, et al. Unintrusive multi-cancer detection by circulating cell-free DNA methylation sequencing (THUNDER): development and independent validation studies[J]. Annals of Oncology, 2023. 版权声明 本网站所有注明“来源:生物谷”或“来源:bioon”的文字、图片和音视频资料,...
Clinical validation of a multicancer detection blood test by circulating cell-free DNA (cfDNA) methylation sequencing: The THUNDER study.doi:10.1200/JCO.2022.40.16_suppl.10544544333210544#Background:The development of ultra-sensitive genomic and epigenomic assays enables the early detection of ...
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