Abstract 7024: Development of quality control methods for reliable NGS measurement of methylation in cfDNAdoi:10.1158/1538-7445.AM2024-7024Background and Purpose: Reliable measurement of circulating free genomic DNA (cfDNA) methylation patterns promises a means for cancer early detection as well as ...
copy number, and fragmentomic patterns in a single assay. Using a total of 950 plasma samples (361 healthy controls and 107 colon, 113 liver, 238 lung, and 131 prostate cancer patients) and 240 tissue samples, we generated whole-genome methylation sequencing (WGMS) data,...
Therefore, methylation profile, a widely used epigenetic biomarker, can be obtained from a single WGS assay for MCED.Introduction Cell-free DNA fragmentomics is a rapidly developing emerging field with significant biological and clinical implications. Recently, it has been mainly used as an ...
2021年7月,清华大学自动化系的汪小我教授团队在Briefings in Bioinformatics上发表了题为“DISMIR: deep learning-based non-invasive cancer detection by integrating DNA sequence and methylation information of individual cell-free DNA re...
methylation enrichment, which removed the need for filler DNA (as used by Huang et al.32) and enabled efficient analysis of samples at low cfDNA inputs (down to 1 ng). Ligated DNA was pooled (to a minimum of 75 ng in total) and combined with 0.3 ng of control methylated ...
Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease. Nat Commun. 2018;9(1). Grabuschnig S, Bronkhorst AJ, Holdenrieder S, Rodriguez IR, Schliep KP, Schwendenwein D, et al. Putative origins of cell-free DNA in humans: a ...
Cell free DNA (cfDNA)-based assays hold great potential in detecting early cancer signals yet determining the tissue-of-origin (TOO) for cancer signals remains a challenging task. Here, we investigated the contribution of a methylation atlas to TOO detec
2G). The findings suggest that variations in hydroxylmethylation levels of specific genes could act as indicators for the emergence of metastasis in individuals diagnosed with ESCC. Fig. 2 Predictive models for detection metastasis of ESCC by 5hmC biomarkers in cfDNA. A Schematic of the machine ...
2021年7月9日,清华大学自动化系的汪小我教授团队在Briefings in Bioinformatics上发表了题为“DISMIR: deep learning-based non-invasive cancer detection by integrating DNA sequence and methylation information of individual cell-free DNA reads”的文章。基于深度学习方法,该研究实现了通过血浆cfDNA的WGBS数据进行高...
These strategies are either very targeted, as methylation events of interest occur at known, stereotyped positions [117], or larger to identify methylation patterns, which have been shown to enable accurate determination of cell-of-origin from cfDNA and non-invasive cancer classification. For example...