next-generation sequencingMethylation signatures in cell-free DNA (cfDNA) have shown great sensitivity and specificity in the characterization of tumour status and classification of tumour types, as well as the response to therapy and recurrence. Currently, most cfDNA methylation studies are based on ...
Multimodal epigenetic characterization of cell-free DNA (cfDNA) could improve the performance of blood-based early cancer detection. However, integrative profiling of cfDNA methylome and fragmentome has been technologically challenging. Here, we adapt an enzyme-mediated methylation sequencing method for com...
Here, we present an integrated experimental and computational system for the accurate and affordable detection of cancer. It consists of (1) a cost-effective experimental assay, named cell-free DNA Methylome Sequencing (cfMethyl-Seq), for genome-wide methylation profiling of cfDNA, and (2) a co...
Methylated DNA fragments from all plasma samples were precipitated and subjected to paired-end DNA sequencing as described before26(Methods). We obtained a median of 63 million reads, covering over 60% of the genome, and showed enrichment of CpGs (Methods) between 1.59 and 2.19 fold (Supplementa...
Ultra Low RNA Sequencing Digital RNA Sequencing (UMI-RNA-Seq) Dual RNA-seq microRNA Sequencing Service mRNA Sequencing Service Epigenomics EpiTYPER DNA Methylation Analysis Targeted Bisulfite Sequencing Reduced Representation Bisulfite Sequencing Whole Genome Bisulfite Sequencing (WGBS) MeDIP Sequencing (Me...
(TF) and limited number of DNA fragments restricts low-disease-burden monitoring through the prevailing deep targeted sequencing paradigm. We reasoned that breadth may supplant depth of sequencing to overcome the barrier of cfDNA abundance. Whole-genome sequencing (WGS) of cfDNA allowed ultra-...
Four healthy individuals volunteered plasma for cell-free DNA analysis. (B) Experimental workflow. cfDNA is extracted from plasma, and whole-genome bisulfite sequencing is performed. In parallel, methylation profiles of cell and tissue genomes are obtained from publicly available databases. cfDNA ...
based screening approach using cell-free DNA (cfDNA) for early detection of hepatocellular carcinoma (HCC).Differentially, DNA methylation blocks were determined by comparing methylation profiles of biopsy-proven HCC, liver cirrhosis, and normal tissue samples with high throughput DNA bisulfite sequencing...
Cell-free RNAs (cfRNAs) offer an opportunity to detect diseases from a transcriptomic perspective, however, existing techniques have fallen short in generating a comprehensive cell-free transcriptome profile. We develop a sensitive library preparation me
2023年5月发表在Genome Medicine上的文章“Single-molecule methylation profiles of cell-free DNA in cancer with nanopore sequencing”开发了一种使用基于纳米孔的单分子测序来检测cfDNA甲基化的模型。用此模型对癌症患者的cfDNA甲基化水平进行定义,用来区分cfDNA片段是来自肿瘤还是免疫细胞,以便在治疗期间进行纵向监测...