While MuSC clusters 1 and 2 are very similar in their gene expression profiles, there are certain genes and pathways that differentiate the two (Fig. 2c, e, f). MuSC Cluster 1 is also reduced in aging, albeit to a lesser extent than Cluster 3 (Fig. 1e). MuSC Cluster 1 is character...
Collaboration between these two signaling pathways is effective in bone repair, but no information exists regarding their combined systemic impact (Lee et al., 2015). In fact, EGFR activation targets the skeleton as it induces proliferation of osteogenic precursors, as occurs in DT/DTRADQ mice (...
the intrinsic nature of ECs interacting with other neural cell types to recapitulate brain vascular morphogenesis during developmental process via vasculogenesis [47,48], in which immature ECs recruit PCs and ACs to form new vessels through PDGFR and Sonic hedgehog (SHH) signaling pathways [47,49]...
Subsequently, the cell-cell communication network was constructed using the netAnalysis_signalingRole_scatter function. Signaling groups based on functional or structural similarity were identified using the “computeNetSimilarity” function. Further analyses included the calculation of outgoing and incoming ...
4b). Moreover, using this replication set, we observed similar under-expression of PMS-related gene sets implicated broad early developmental pathways as well as ECM-related terms and Wnt-signaling (Supplemental Table 4). Fig. 4 Replication of hiPSC-neuron RNA-seq. A replication set of hiPSC...
To remove cells of poor quality, we used scater [103] to compute QC metrics. For each barcode, we assessed the total UMI counts, total number of genes detected, and the proportion of counts mapping to mitochondrial genes (Additional file 1: Fig. S2A). We removed any barcodes that deviat...
The analyses revealed an extensive cohort of PDGF-dependent biological processes and connected key transcriptional effectors to their regulation, significantly expanding current knowledge of PDGF-stimulated signaling cascades. These observations also implicate MYC as a novel target for pharmacological ...
Growth factor-based medium is a major focus of hiPSC research, and most popular commercial media, such as mTeSR1 and E8, contain the two factors FGF2 and TGFβ, which drive hPSC pluripotency by activating PI3K/AKT/mTOR pathways and TGFb signaling pathways, respectively, but the high cost of...
signaling pathways as a response to exercise. But if they remain in adormant statefor too long, they can become damaged as they fill with cellular trash. In their dormant state, they still produce such trash, but because they are dormant, they cannot expel it as most cells normally do. ...
NF-κB and Hypoxia signaling are intimately linked to chemoresistance through regulation of cell proliferation and survival in several cancers, including breast cancer37,38,39,40. Next, we found activation of NF-κB, hypoxia and Notch pathways enriched in Dll1+ tumor cells (Fig. 4c and ...