we adoptively transferred sorted naive LATG135DCD4 T cells labeled with CellTrace Violet proliferation dye into sublethally irradiated congenic CD45.1+C57BL/6 hosts to examine the homeostatic proliferation. After 4 days, we observed that LATG135DCD4 T cells proliferated more robustly...
Live HeLa cells labeled with Tubulin Tracker Deep Red and NucBlue Live ReadyProbes Reagent, enabling visualization of the microtubule cytoskeleton and nuclei in live cells, respectively. High resolution images were acquired using a confocal s...
When the N-GR expressing tissue-resident cell lines were co-cultured with cancer cells expressing sC-GR for 24 h at a ratio of 1:1, fluorescent confocal microscope observation and flow cytometry analysis confirmed that the tissue-resident cells were selectively labeled with GFP at high ...
scratches were made in the confluent monolayers using a pipette tip, followed by washing the wounds twice with PBS. Images of cells migrating to the wound area at both 0 and 24 h were captured using a phase-contrast microscope (Nikon). Finally, the distance ...
The reported and newly identified regulatory genes under the single-cell resolution in this study based on gene differential expression, cell trajectory inference, RNA velocity fate determination, and co-expression gene interaction are labeled in the corresponding module. The red oval represents the ...
Staining and examination of the slides under a fluorescence microscope can be performed in less than one hour. Each Cell Culture Contamination Detection Kit contains: Green-fluorescent SYTO 9 nucleic acid stain Blue-fluorescent Cal...
These results strongly suggest that these cells are broken but not visible as such under the microscope. Therefore, they need to be treated as low quality and excluded from downstream analysis. Identification of low quality cells After curating a set of mESC specific and common features, our aim...
[22,23], which however, requires specific imaging modeling. Reconstruction can also be built into active contours with constrained iterative deconvolution without explicitly computing the inverse problem [24,25]; however, it requires the point-spread function of a microscope, which is measured or ...
The three most stable states according to the coarse-grained transition matrix were alpha (SI 0.97), beta (SI 1.00) and epsilon (SI 0.98) macrostates, which were accordingly labeled as terminal by CellRank, consistent with known biology (Fig.2d). We recovered one relatively stable (SI 0.84)...
After culture for 7–9 days, the colonies were fixed with methyl alcohol and stained with 0.1% crystal violet for 20 min, and the number of colonies was counted under the microscope (Nikon, Japan). Luciferase reporter assay Luciferase reporter assays were performed with Secrete-Pair Dual ...