7.2 Cell-culture based models Several cell-culture based models suitable to study the interaction between C. acnes (biofilms) and the human host are also available. As acne is an exclusively human disease, these models require the use of human cell lines [111,112]. The skin is the first ...
OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell transplantation in treatment of β-ray-irradiated skin injury in rats. METHODS: Three Sprague-Dawley female rats were killed to isolate and culture the bone marrow mesenchymal stem cells, then the cells were labeled with 4'...
As visualized by 3D LSCM imaging, dense OMVs labeled with N-hydroxysuccinimide (NHS) ester-functionalized Cy5 fluorescent dye (Cy5-NHS) were dispersed uniformly inside the network (Fig. 2L), which verified that SFSHs with a biomimetic cell-compartmentalized structure to the skin were formed ...
Briefly, each single cell is isolated with an elongated Pasteur pipette, under direct vision using an inverted microscope, before it is carefully deposited at the centre of a 35 mm Petri dish containing lethally irradiated 3T3 cells. After seven days of cultivation, each dish is carefully ...
Live cell measurements were performed using a Bruker BioScope Catalyst AFM system (Bruker, Santa Barbara, CA) mounted on an inverted Axiovert 200 M microscope system (Carl Zeiss, Göttingen, Germany) equipped with a Confocal Laser Scanning Microscope 510 META (LSM 510 Meta, Carl Zeiss) and...
You won’t actually see the dust mites that share your home, because they’re vanishingly small (a family of mites could pack themselves into the period at the end of this sentence). But if you looked at one under a microscope, you’d see an otherworldly, eight-legged creature. ...
These cells need to be passaged at a very low density, under which they maintain a basal cell-like phenotype. Once these cells reach a high enough density or are exposed to other differentiation signals, they can lose their ability adhere and proliferate. As a brief technical note, we have...
Furthermore, compared to fluorescence microscopy, Raman spectroscopy does not require the molecules to be labeled and the spectrum emitted is entirely dependent on the biochemical composition of the observed voxels, which is performed without damaging the cell environment or labeling other samples.34,35...
The presence of mononuclear cells coexpressing these antigens was demonstrated by three techniques: double labelling immunofluorescence using FITC and rhodamine conjugated goat antimouse IgG; fluorescence activated cell sorter (FACS); and by direct counting (under the microscope) of cells stained by ...
All images were evaluated under a fluorescence microscope (Olympus, Tokyo, Japan). The percentage of TFEB- and LC3-positive cells in the dermal layer was calculated. Six random fields of three random sections from each tissue sample were used....