Live cell measurements were performed using a Bruker BioScope Catalyst AFM system (Bruker, Santa Barbara, CA) mounted on an inverted Axiovert 200 M microscope system (Carl Zeiss, Göttingen, Germany) equipped with a Confocal Laser Scanning Microscope 510 META (LSM 510 Meta, Carl Zeiss) and...
The negative control samples were used to define the range of live cells, and this gate was applied to all other samples to determine the percentage of cells with PKH26-labeled EVs. 2.9. RAW-Blue™ NF-κβ reporter assay RAW-Blue™ cells (murine RAW 264.7 macrophage reporter cell line...
ideal choice to detect the cell environment at the molecular level and is unaffected by factors such as oxygen, temperature and water. Furthermore, compared to fluorescence microscopy, Raman spectroscopy does not require the molecules to be labeled and the spectrum emitted is entirely dependent on ...
The sections were then incubated with corresponding secondary antibodies labeled with Alexa Fluor 488, 594, and 647. Nuclear counterstaining was performed with Hoechst. Stained tissues were imaged with a Zeiss confocal microscope (LSM-880) (Carl Zeiss, Oberkochen, Germany). In the dermis, positive...
As visualized by 3D LSCM imaging, dense OMVs labeled with N-hydroxysuccinimide (NHS) ester-functionalized Cy5 fluorescent dye (Cy5-NHS) were dispersed uniformly inside the network (Fig. 2L), which verified that SFSHs with a biomimetic cell-compartmentalized structure to the skin were formed ...
OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell transplantation in treatment of β-ray-irradiated skin injury in rats. METHODS: Three Sprague-Dawley female rats were killed to isolate and culture the bone marrow mesenchymal stem cells, then the cells were labeled with 4'...
The factors responsible have not been fully delineated in humans [1,2], although there are gene expression data from partial-thickness excisional and burn wounds [3,4] as well as full-thickness skin wounds in patients with basal cell carcinoma [5]. The suction-blister wound healing model is...
Cell traffic was recorded for 1.5 h, 90-sec step in time, starting 30 min after the addition of tested compounds, using DMI 6000B microscope (Leica). Data are presented in diagrams, in which the starting point of individual cell trajectory is in the center of the diagram. Analysis of ...
These cells need to be passaged at a very low density, under which they maintain a basal cell-like phenotype. Once these cells reach a high enough density or are exposed to other differentiation signals, they can lose their ability adhere and proliferate. As a brief technical note, we have...
These cells need to be passaged at a very low density, under which they maintain a basal cell-like phenotype. Once these cells reach a high enough density or are exposed to other differentiation signals, they can lose their ability adhere and proliferate. As a brief technical note, we have...